感染丝状蓝藻的噬藻体的裂解周期和释放量的测定  

Measurement of Lysing Cycle and Burst Size of Cyanophage Infecting Filamentous Cyanobacteria(blue-green algae)

作　　者：程凯[1] 王春艳[1] 郭亚新[1] 石正丽[2] 赵以军[1] 

机构地区：[1]华中师范大学生命科学学院,湖北武汉430079 [2]中国科学院武汉病毒研究所无脊椎动物病毒学联合开放实验室,湖北武汉430071

出　　处：《中国病毒学》2002年第4期374-376,共3页Virologica Sinica

基　　金：国家自然科学基金资助 (3 960 0 0 0 4;3 9970 0 64 7)

摘　　要：Several methods were tested for measurement of lysing cycle and burst size of the cyanophage infecting the filamentous cyanobacterium \%Plectonema boryanum\% IU594. The results indicated that the lysing curve of host cells was consistent with the one step growth curve of cyanophage, thus it was more convenient to substitute measuring the lysing curve of host cells for the one step growth curve measurement of cyanophage. Besides,the burst size increased with the reducing cyanophage inoculation, reaching the maxium of 206PFU/Cell by 1PFU infection. It was suggested that more exact burst size of cyanophage could be obtained under 1PFU cyanophage inoculation.Several methods were tested for measurement of lysing cycle and burst size of the cyanophage infecting the filamentous cyanobacterium \%Plectonema boryanum\% IU594. The results indicated that the lysing curve of host cells was consistent with the one step growth curve of cyanophage, thus it was more convenient to substitute measuring the lysing curve of host cells for the one step growth curve measurement of cyanophage. Besides,the burst size increased with the reducing cyanophage inoculation, reaching the maxium of 206PFU/Cell by 1PFU infection. It was suggested that more exact burst size of cyanophage could be obtained under 1PFU cyanophage inoculation.

关 键 词：丝状蓝藻 噬藻体 裂解周期 释放量 测定 浮游病毒 

分 类 号：Q935[生物学—微生物学]