反相高效液相色谱法测定无花果中游离氨基酸的含量  

Determination of free amino acid contents in Ficus carica by RP-HPLC

作　　者：席荣英[1] 李聪辉[1] 孙祥德[1] 齐伟[1] 

机构地区：[1]河南省新乡医学院化学教研室,河南新乡453003

出　　处：《新乡医学院学报》2002年第5期382-383,共2页Journal of Xinxiang Medical University

摘　　要：目的　测定无花果中游离氨基酸的含量。方法　用OPA与 2 -ME作衍生剂 ,在梯度洗脱、荧光检测下 (λex =335nm ,λem =4 30nm) ,采用反相高效液相色谱法测定 15种游离氨基酸的含量。结果　无花果果实中含有多种人体必须氨基酸 ,其含量占总游离氨基酸的4 0 .2 0 %。其中 ,丙氨酸、丝氨酸、天门冬氨酸和赖氨酸含量较高。结论　无花果果实是富含氨基酸的物质 。Objective To determine the contents of free amino acids in fresh Ficus carica fruit.Methods Concentrations of 15 kinds of amino acids in Ficus carica fruit were determined by reversed-phase high performance liquid chromatography(RP-HPLC),with OPA and 2-mercapyo ethanol as precolumn derivative reagents.Gradient elution and fluorescence detector was used(λex=335nm,λem=430nm).Results Fifteen kinds of amino acids had been found, and there were many kinds of essential amino acids needed by human body in Ficus carica fruit.The contents of essential amino acids in the total free amino acids were 45.06% and concentrations of Ala, Ser, Lys and Asp were slightly higher.Conclusion Amino acids are rich in Ficus carica fruit which is valuable as special diet.

关 键 词：无花果 氨基酸 反相高效液相色谱法 含量测定 

分 类 号：R284[医药卫生—中药学] R282.5[医药卫生—中医学]