缺血预处理对沙土鼠前脑缺血再灌注后海马羟自由基生成的影响  

THE EFFECT OF ISCHEMIC PRECONDITIONING ON HIPPOCAMPAL HYDROXYL RADICAL LEVELS IN GERBILS AFTER CEREBTRAL ISCHEMIA/REPERFUSION

作　　者：王春林[1] 许鹏程[2] 曾因明[2] 袁丽丽 范建伟[2] 于常州[2] 

机构地区：[1]江苏省苏北人民医院,江苏扬州225001 [2]江苏省麻醉学重点实验室,江苏徐州221002

出　　处：《江苏临床医学杂志》2002年第5期414-416,共3页Journal of Jiangsu Clinical Medicine

基　　金：江苏省卫生厅科研基金资助课题 (H990 8)

摘　　要：目的 :研究缺血预处理对沙土鼠脑缺血再灌注后海马羟自由基含量的影响 ,以探讨其神经保护作用的可能机制。方法 :沙土鼠前脑缺血再灌注模型 :阻断双侧颈总动脉 5min ,然后松开即再灌注。将沙土鼠随机分为假手术组 (Sh组 )、缺血组(Is组 )、短暂缺血组 (Po组 )和缺血预处理组 (Ip组 ) ,每组 6只。在动物处死前 3 0min腹腔注射水杨酸钠 10 0mg/kg。在再灌注60min ,快速断头取脑 ,在冰面上分离海马 ,用高效液相电化学检测方法测定各组沙土鼠海马 2 ,3 -二羟基苯甲酸 (2 ,3 -DH BA)、2 ,5 -二羟基苯甲酸 (2 ,5 -DHBA)的含量 (mmol/L)。结果 :沙土鼠脑缺血再灌注 60min ,Sh、Is、Po和Ip组海马中 2 ,3 -DHBA、2 ,5 -DHBA含量分别为 0 2 3± 0 0 5、0 73± 0 12 ;0 46± 0 0 4、1 2 3± 0 11;0 2 7± 0 0 4、0 80± 0 12 ;0 2 9± 0 0 3、0 87± 0 0 7(mmol/L)。Is、Po和Ip组海马中 2 ,3 -DHBA、2 ,5 -DHBA含量分别约为Sh组 2 0 3 %、169% (P <0 0 1) ;118%、110 % (P >0 0 5 ) ;13 1%、119% (P >0 0 5 )。与Is组相比 ,Ip组海马中 2 ,3 -DHBA、2 ,5 -DHBA含量分别减少约 3 7% (P<0 0 1)和 2 9% (P <0 0 1) ;Po组海马中 2 ,3 -DHBA、2 ,5 -DHBA含量分别减少约 41% (P <0 0 1)和 3 5 % (PObjective:To investigate the effect of ischemic preconditioning on hippocampal hydroxyl radical levels in gerbils after cerebral ischemia/reperfusion.Methods:Forebrain ischemia in gerbils was produced by occlusion of bilateral common carotid arteries using miniature aneurysmal clips for 5 min.30 gerbils were randomly divided into 4 groups:Sham-operated group(Sh group),Ischemic group (Is group),preconditioning only group (Po group),ischemic preconditioning group (Ip group,double 2-min ischemic treatments at 24 h intervals,48 h before 5 min ischemia).The hippocampal hydroxyl radical levels were measured using high pressure liquid chromatography (HPLC) with electrochemical detection(ECD) at 60 min of ischemic reperfusion.Results:The levels of hippocampal 2,3-DHBA and 2,5-DHBA in Po group and Ip group had no significant difference compared with that in Sh group(P>0.05).The levels of hippocampal 2,3-DHBA and 2,5-DHBA in Is group were 0.46±0.04mmol/L and 1.23±0.11mmol/L,respectively,much more than that in Sh group 0.23±0.05mmol/L and 0.73±0.12mmol/L.The levels of hippocampal 2,3-DHBA and 2,5-DHBA in Is,Po, and Ip group were 203% (P<0.01) and 169% (P<0.01),118% (P>0.05) and 110%(P>0.05),131%(P>0.05) and 119%(P>0.05),of that in Sh group,respectively.Compared with that in Is group, the levels of hippocampal 2,3-DHBA and 2,5-DHBA in Ip group were decreased by 37%(P<0.01) and 29%(P<0.01),and that in Po group were were decreased by 41%(P<0.01) and 35% (P<0.01) respectively.Conclusion:Ischemic preconditioning can significantly prevent the generation of hippocampal hydroxyl radical after cerebral ischemia/reperfusion.

关 键 词：脑缺血 缺血预处理 海马 羟自由基 氧自由基 

分 类 号：R743.3[医药卫生—神经病学与精神病学]