脑梗死患者血清白介素-6、白介素-8、肿瘤坏死因子-α及白介素-1β水平的研究  

Detection of the levels of serum IL-6,IL-8,TNF-α,and IL-1β in patients with acute cerebral infarction.

作　　者：崔丽[1] 郑惠民[1] 陶沂[1] 王晔[1] 张仁琴[1] 

机构地区：[1]第二军医大学第一附属医院神经内科,上海200433

出　　处：《中国综合临床》2002年第12期1083-1084,共2页Clinical Medicine of China

摘　　要：目的研究脑梗死患者急性期血清 IL -6、IL-8、TNF-α、IL -1β水平的变化 ,特别是 IL -6水平与脑梗死面积及神经功能缺损的关系。方法采用双抗体夹心 ELISA法检测 3 4例脑梗死急性期患者不同时间内血清 IL-6、IL -8、TNF-α、IL -1β水平 ,并随机抽取 10例健康人进行对照。结果脑梗死患者血清 IL -6水平在发病 6小时内明显升高 ,2 4～ 3 6小时达高峰 ,7天后基本降至正常 ,大面积梗死组IL-6水平明显高于小面积梗死组 ( 6小时内 ,P <0 .0 5 ;2 4～ 3 6小时 ,P <0 .0 5 ) ,且血清 IL -6水平与神经功能评分呈正相关( r=0 .87,P<0 .0 1) ,七叶皂甙钠治疗 3天后血清 IL-6水平下降明显 ( P<0 .0 1) ,脑梗死患者急性期血清 IL-8水平亦升高( P<0 .0 5 ) ,TNF-α、IL-1β水平则无明显变化。结论急性脑梗死患者血清 IL-6水平升高 ,而 IL-6升高的水平反映应激反应程度 ,与梗死面积及神经功能缺损程度有一定相关性。objective To investigate the changes of serum IL 6,IL 8,TNF α,and IL 1β,and in particular,the relationship between IL 6 and infarct volume and neurological function deficit in patients with acute cerebral infarction.Methods ELISA was applied to measure serum levels of IL 6,IL 8,TNF α,and IL 1β in 34 cerebral infarction patients in different time.10 healthy subjects were selected randomly as control group.Results Serum levels of IL 6 showed a significant increase within 6 hours following the onset and peaked at 24～36 h,which returned to normal 7 days later.Serum levels of IL 6 in patients with large lesion were higher than those with small lesion(in 6 h,P<0.05; at 24～36 h,P<0.05).In addition,serum levels of IL 6 was positively correlated with neurological function scoring(r=0.87,P<0.01).A clear decline of IL 6 level was seen in 3 days treatment with sodium escin (P<0.01) but an increase of IL 8 was present in the patients at the acute phase(P<0.05).However,TNF α and IL 1β were unchangable(P<0.01).Conclusion The serum levels of IL 6 in the patients with acute cerebral infarction is elevated.Such level of elevation of IL 6 releflects the degree of stress and is correlated with the infarct volume and neurological deficit.

关 键 词：白介素-6 白介素-8 肿瘤坏死因子-Α 白介素-1Β 脑梗死 细胞因子 七叶皂甙钠 神经功能 双抗体夹心酶联免疫吸附法 

分 类 号：R743.33[医药卫生—神经病学与精神病学]