大鼠中缝背核一氧化氮合酶神经元投射分布于大脑皮质一氧化氮合酶神经元  

Project of Dorsal Raphe Nucleus Nitric Oxide Synthase(NOS) Containing Neurons to NOS Containing Neurons in the Rat Cerebral Cortex

作　　者：王生福[1] 姜平[1] 童鑫康[1] 韩群颖[2] 

机构地区：[1]江苏大学医学院人体解剖学教研室,江苏镇江212001 [2]南京医科大学人体解剖学与组织胚胎学系,江苏南京210029

出　　处：《江苏大学学报（医学版）》2002年第5期433-435,I001,共4页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省卫生厅资助项目 (H972 6)

摘　　要：目的 :研究通过损毁脑干中缝背核 (DR) ,探讨中缝背核一氧化氮合酶 (NOS)阳性神经元是否投射分布于大脑皮质NOS阳性神经元。方法 :将 16只SD雄性成年大鼠分为实验组与对照组。对实验组动物DR微量注射喹啉酸 ,饲养 1周 ,灌注固定 ,然后将大脑及脑干作冠状冷冻切片 ,NADPH d组化染色。结果 :实验组动物的中缝背核被有效损毁 ,其NOS阳性神经元的数量减少了5 9 .1% (P <0 .0 0 1)。大脑皮质NOS阳性纤维终末减少了 2 8.2 % (P <0 .0 5 ) ,与大脑皮质NOS神经元构成接触的NOS阳性纤维终末减少了 3 3 .9% (P <0 .0 5 )。结论 :位于中缝背核的NOS阳性神经元投射分布于大脑皮质NOS神经元。推测中缝背核的NOS阳性神经元可能通过大脑皮质NOS阳性神经元间接对皮质脑血流量起调节作用。Objective: To study the NOS containing fibers from the dorsal raphe nucleus projecting to NOS containing neurons in the cerebral cortex. Methods:16 male adult S.D. rats were divided into two groups. The experimental group was microinjected with quisqualic acid and then raised for a week. These rats were perfusion fixed, and the brain were sectioned and stained by the NADPH d histochemistry.Results:The NOS positive neurons in dorsal raphe nucleus were decreased significantly by 59.1% ( P <0.001). The NOS positive terminals were decreased by 28.2%( P <0.05) in the cerebral cortex, and the NOS positive terminals associating with the NOS containing neurons in the cerebral cortex were decreased by 33.9%( P <0.05).Conclusion:It is suggested that the NOS containing neurons in dorsal raphe nucleus projected to the NOS containing neurons in cerebral cortex.

关 键 词：一氧化氮 NADPH-d组织化学 NOS阳性神经元 大脑皮质 中缝背核 大鼠 

分 类 号：R－332[医药卫生] R338