肿瘤患者红细胞天然免疫分子CD35和趋化因子受体的变化  

ANALYSIS OF CHANGES IN CD35 AND CKR ON RED BLOOD CELLS IN PATIENTS WITH CARCINOMA

作　　者：郭峰[1] 张乐之[1] 钱宝华[1] 花美仙[1] 许育[1] 薛春燕[1] 张军[1] 王雅杰[1] 

机构地区：[1]第二军医大学长海医院

出　　处：《解放军医学杂志》2002年第11期971-972,共2页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 39670 754)

摘　　要：采用流式细胞仪测定红细胞CD35 (RBC CD35 ) ,用免疫酶联法测定红细胞趋化因子受体 (ECKR) ,研究肿瘤患者红细胞CD35与趋化因子受体的变化规律。结果显示 ,2 5例肿瘤患者红细胞CD35数量 (41 0 2± 14 86 )、ECKR活性 (46 0 5± 13 33)明显低于 2 9例正常人的相应指标水平 (5 3 39± 39 2 2和 5 3 5 8± 10 2 6 ,P <0 0 5 )。肿瘤患者血清细胞介素 8(sIL 8)含量 (3 37± 2 5 9)高于正常人(2 92± 1 0 7) ,鼻咽癌患者RBC CD35数量 (2 6 0 6± 3 0 0 )明显低于其他肿瘤患者 (38 90～ 5 2 4 7,P <0 0 5 )。提示肿瘤患者红细胞天然免疫分子与sIL 8之间存在一定的相关性 。To research the regularity of changes of RBC CD35 and ECKR in patients with carcinoma, studies of CD35 on red blood cells(RBC) using flow cytometry and ECKR and sIL 8 using ELISA were carried out in patients with carcinoma. It was found that number(41 02±14 861) of RBC CD35 and activity (46 05±13 33) of ECKR in 25 patients with carcinoma were significantly lower than those (53 39±39 22 and 53 58±10 26) in normal people ( P <0 05). The numbers (3 37±2 59) of sIL 8 in patients with carcinoma was larger than that (2 92±1 07) in normal people,and the number(26 06±3 00) of RBC CD35 in patients with nasopharyngeal cancer was significantly lower than that ( =38 90-52 47) in patients with other carcinoma ( P <0 05). Our conclusions are there is a relation between activity of RBC innate immune molecules and number of sIL 8 in patients with carcinoma. The analysis of changes in RBC innate immune molecule can be helpful to exploring regulatory pattern of immune reaction, and at the same time get applicable information in clinical medicine.

关 键 词：肿瘤 红细胞CD35 红细胞趋化因子受体 天然免疫分子 血清细胞介素8 细胞因子 免疫功能 流式细胞术 免疫酶联竞争法 

分 类 号：R73[医药卫生—肿瘤]