134例结核杆菌5种耐药基因的检测  

THE RESEARCH OF FIVE DRUG-RESISTANCE GENES OF MYCOBACTERIUM TUBERCULOSIS IN 134 PATIENTS

作　　者：李洪敏[1] 吴雪琼[1] 王巍[1] 曹利雪[1] 李素梅[1] 肖漓[1] 

机构地区：[1]解放军第309医院

出　　处：《解放军医学杂志》2002年第11期993-994,共2页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科研"十五"规划面上项目 (编号 0 1MA2 1 7);国家"十五"科技攻关基金 (编号 2 0 0 1BA70 5B0 4 )资助课题

摘　　要：应用聚合酶链反应 单链构象多态性 (PCR SSCP)方法分析 134例临床分离株的 5种结核杆菌 (MT)耐药基因突变情况。结果表明 ,耐吡嗪酰胺 (PZA)pncA基因 ,耐链霉素 (SM)rpsL基因 ,耐利福平 (REP)rpoB基因 ,耐异烟肼 (INH)katG基因 ,耐乙胺丁醇 (EMB)embB基因的突变率分别为 4 2 7% ,71 7% ,78 9% ,6 8 6 % ,4 3 9%。证实MT存在多种耐药基因突变的情况。但MT药敏实验与 5种耐药基因突变率并不一致 ,说明MT的耐药除基因突变外 。M.tuberculosis strains isolated strains from sputum specimens of 134 patients were analyzed by PCR SSCP and traditional drug susceptibility tests. The results sbowed that the gene mutation rate of PZA, SM, RFP,INH and EMB resistance in those clinically isolated strains was 42 7%,71 7%,78 9%,68 6% and 43 9%, respectively. The gene mutation was in relation with drug resistance level of M. tuberculosis. The gene mutation rate was higher in high concentration resistance strains than in low concentration resistance strains.

关 键 词：结核杆菌 耐药基因 分枝杆菌 药物耐受性 聚合酶链反应-单链构象多态性 药敏试验 

分 类 号：R378.911[医药卫生—病原生物学] R91[医药卫生—基础医学]