Gαq/11介导的信号转导通路在自发性高血压大鼠主动脉中的变化  

Change of Gαq/11-mediated signal transduction pathway in aorta of spontaneously hypertensive rats

作　　者：王瑾瑜[1] 白桦[2] 邢东琦[1] 刘洁[1] 吴立玲[1] 

机构地区：[1]北京大学医学部生理与病理生理学系,北京100083 [2]邮电总医院心内科,北京100032

出　　处：《中国病理生理杂志》2002年第11期1313-1316,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (30 1 70 379;39870 356)

摘　　要：目的 :观察自发性高血压大鼠 (SHR)主动脉Gαq/ 1 1及磷脂酶C(PLC)的动态变化 ,探讨其在SHR高血压发病机制中的意义。方法 :4周龄和 1 2周龄SHR ,颈动脉插管记录动脉血压 ,放免法测定血浆血管紧张素Ⅱ的浓度 ,免疫印迹法检测主动脉组织Gαq/ 1 1和PLC的含量。结果 :SHR 1 2周龄时动脉血压明显增高。 4周龄SHR主动脉Gαq/ 1 1的表达较对照高 69 2 % (P <0 0 5)。 4周龄和 1 2周龄SHR主动脉PLCβ3分别较各自同龄对照组高66 9%和 85 1 % (P <0 0 5)。结论 :Gαq/ 1AIM: To investigate the changes of Gαq/11 and phospholipase C(PLC) of the aorta and to evaluate the role of signal transduction pathway mediated by Gαq/11 in the pathogenesis of spontaneous hypertension. METHODS: Blood pressure was measured by intubation of carotid, and the level of plasma angiotension Ⅱ was measured by radioimmunoassay. In addition, Gαq/11 and PLC contents in aorta were determined by Western blot analysis. RESULTS: Arterial blood pressure was not changed in 4-week-old spontaneously hypertensive rats(SHR), but it was increased markedly in 12-week-old SHR. The Gαq/11 expression of aorta in 4-week-old SHR was increased by 69 2%( P<0 05 ) The levels of PLCβ 3 of aorta in 4-and 12-week-old SHR were increased by 66 9% and 85 1%( P<0 05 ), respectively, compared with their age-matched WKY rats.CONCLUSION: The up-regulation of Gαq/11-mediated signal transduction pathway of aorta may contribute to the initiation and maintenance of spontaneous hypertension.

关 键 词：Gαg/11 信号转导通路 自发性高血压 大鼠 主动脉 发病机制 放射免疫法 免疫印迹法 

分 类 号：R544.1[医药卫生—心血管疾病]