低温对大鼠血浆及不同脑区神经肽等活性物质含量变化的影响  

Effects of hypothermia on the contents of neuropetides and other active substances in rat plasma and different brain regions

作　　者：薛秀兰[1] 邢凤有[1] 王淑秋[1] 李守文[1] 

机构地区：[1]黑龙江省佳木斯大学基础医学院病理生理学教研室,黑龙江佳木斯154003

出　　处：《中国病理生理杂志》2002年第11期1411-1414,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨低温对血浆及不同脑区精氨酸加压素 (AVP)、血管紧张素Ⅱ (AngⅡ )、亮氨酸 -脑啡肽 (L-EK)、环磷酸腺苷 (cAMP)、Ca2 + 及Mg2 + 含量影响。方法 :复制大鼠实验性低温模型 ,随机分组处理 ,实验结束时 ,取下丘脑、脑干及血样品 ,采用放射免疫法测定AVP、AngⅡ、L -EK、cAMP的含量 ,原子吸收法测定Ca2 + 及Mg2 + 含量。结果 :实验组L -EK、cAMP、Ca2 + 及Mg2 + 值显著高于对照组 (P <0 0 1 ) ;实验Ⅰ组、Ⅲ组血浆AVP、AngⅡ值显著高于对照组 (P <0 0 1 ) ,Ⅱ组显著低于对照组 (P <0 0 1 )。实验Ⅰ组、Ⅲ组下丘脑和脑干AVP、AngⅡ值显著低于对照组 (P<0 0 1 ) ,Ⅱ组显著高于对照组 (P <0 0 1 )。结论 :低温时 ,L -EK、AVP、AngⅡ、cAMP、Ca2 + 及Mg2 +AIM: To study the effects of hypothermia on the contents of AVP,Ang Ⅱ,L-EK,cAMP,Mg 2+ and Ca 2+ in rat plasma and different brain regions.METHODS: The model of experimental hypothermia rat was established and treated in groups randomly.At the end of the experiment,samples of plasma and braintissues were taken and the contents of L-EK,cAMP,AVP,Ang Ⅱ were determined by RIA methods,Mg 2+ and Ca 2+ by atom absorption methods.RESULTS: The contents of Ang Ⅱ,AVP increased significantly in the plasma of Ⅰ,Ⅲ group( P<0 01 ),while that of group Ⅱ decreased obviously ( P<0 01 );the contents of Ang Ⅱ,AVP decreased in the hypothalamus and truncus encephalicus of Ⅰ,Ⅲ group( P<0 01 ),and that of group Ⅱ increased ( P<0 01 ).CONCLUSION: L-EK,cAMP,AVP,Ang Ⅱ,Mg 2+ and Ca 2+ might be involved in body temperature regulation during hypothermia.

关 键 词：低温 大鼠 血浆 脑区神经肽 放射免疫法 原子吸收法 活性物质 

分 类 号：R363.123[医药卫生—病理学]