松褐天牛性信息素的触角电位和气质联机鉴定  

Identification of Sex Pheromone by GC-EAD and GC-MS in the Pine Sawyer Beetle, Monochamus alternatus

作　　者：钱明惠 黄泽翰[1,2] 方海鹏 黄咏槐 魏纳生[1,2] 黄焕华 

机构地区：[1]广东省森林病虫害生物防治重点实验室,广东广州510520 [2]广东省林业科学研究院,广东广州510520

出　　处：《广东林业科技》2015年第4期1-5,共5页Forestry Science and Technology of Guangdong Province

基　　金：国家林业局948项目"墨天牛性信息素合成与应用技术引进"(2011-4-61);广东省科技计划项目"松褐天牛性引诱剂的剂型研究和示范"(2014A020208034)

摘　　要：利用松褐天牛(Monochamus alternatus)性信息素提早诱杀携带松材线虫(Bursaphelenchus xylophilus)的松褐天牛,可有效控制松材线虫病的传播蔓延和松褐天牛的为害。性行为学观察、"Y"型嗅觉仪试验结果显示,松褐天牛可能存在接触性信息素和短距离性信息素;通过气相色谱和触角电位(GCEAD)、气质联机(GC-MS)分析,鉴定出1种雄性信息素——壬醛。The pine sawyer beetle, Monochamus alternatus, is the main vector of the pine wood nematode, Bursaphelenchus xylophilus, the causal agent of a lethal wilting disease in pines in China. Bioassays using Y-tube olfactometry revealed that females were attracted to volatile compounds produced by males. However, immature males did not seem to release attractants for mature females and immature females did not show any attraction for mature males. A compound produced specifically by mature males was identified as Nonanal from its gas chromatographic retention times(GC-MC), its mass spectrum and the electroantennographicresponses(GCEAD)to Nonanal only from mature female adultts. Here, volatiles were collected from mature male and female M. alternatus. It has been concluded that Nonanal is a male-produced aggregation pheromone of M. alternatus. The possible roles of this pheromone in the chemical ecology of M.alternatus and its potential use in pine wilt disease management are discussed.

关 键 词：松褐天牛 性信息素 交配行为 “Y”型嗅觉仪 气相色谱-触角电位联用 气相色谱-质谱联用 

分 类 号：S763.38[农业科学—森林保护学]