Slug和E-cadherin在非小细胞肺癌恶性胸水细胞中的表达变化  

Slug and E-cadherin expression in non small cell lung cancer

作　　者：李长思[1] 徐爱华[1] 杨杨[1] 李斌[1] 李建华[2] 孙永新[1] 

机构地区：[1]中国医科大学附属第一医院康复医学科 [2]中国医科大学附属一医院病理科,辽宁110001

出　　处：《中国组织化学与细胞化学杂志》2015年第5期393-398,共6页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的研究锌指转录因子Slug、E-cadherin和Vimentin在非小细胞肺癌恶性胸水细胞中的表达。方法应用免疫细胞化学和western blot检测Slug、E-cadherin和Vimentin在非小细胞肺癌胸水中的表达。结果在121例非小细胞肺癌胸水中,Slug、E-cadherin和Vimentin的阳性表达率分别为35.5%(43/121)、71.9%(87/121)和39.71%(48/121),在非小细胞肺癌细胞中Slug和E-cadherin阳性表达率均高于正常胸水,Slug表达的增强通常伴随E-cadherin表达下调及Vimentin表达的增强。结论 Slug与E-cadherin在胸水中非小细胞肺癌细胞中的表达呈负相关,在胸水中提取非小细胞肺癌细胞,研究Slug和E-cadherin,Vimentin的表达及其在上皮间质转化中的调节作用对探讨肿瘤细胞在人体中的转移进展很有意义。Objective To study Slug and E-cadherin expressions in non small cell lung cancer. Methods The expression of Slug,E-cadherin and Vimentin in the lung cancer was detected by immunocytochemistry and Western blotting. Results In the pleural effusion of 121 patients with non small cell lung cancer,the positive expression rates of Slug,E-cadherin and Vimentin were respectively35. 5%( 43 /121),71. 9%( 87 /121),and 39. 71%( 48 /121),and the rates of positive expression of Slug and E-cadherin were higher than in normal plural effusion. The augmentation of Slug expression was usually accompanied by down-regulated E-cadherin expression and enhanced Vimentin expression. Conclusion The expressions of Slug and E-cadherin in plural effusion of non small cell lung cancer are negatively correlated( P < 0. 05). It is very meaningful to extract non small cell lung cancer cells from plural effusion and to study Slug,E-cadherin and Vimentin expressions and their regulatory role in the epithelial mesenchymal transformation in order to explore the metastatic progression of tumor cells in the human body.

关 键 词：SLUG E-CADHERIN EMT 胸腔积液 非小细胞肺癌细胞 

分 类 号：R734.2[医药卫生—肿瘤]