结直肠癌肿瘤实质和肿瘤间质中MMP-2的表达  

Expression of Matrix-metalloproteinase-2 in Colorectal Tumor Epithelial Cells and Mesenchymal Cells

作　　者：骆新兰[1] 蔡秀玲[1] 庄恒国[1] 林华欢[1] 

机构地区：[1]广东省人民医院病理科,510080

出　　处：《实用癌症杂志》2002年第6期614-616,共3页The Practical Journal of Cancer

摘　　要：目的　探讨结直肠癌肿瘤实质及肿瘤间质中基质金属蛋白酶 -2 (MMP -2 )的表达及其病理学意义。方法　采用免疫组化LSAB法对 13 7例结直肠癌进行免疫组化检测 ,取 12例大肠腺瘤样息肉及 8例正常大肠粘膜组织作对照。结果　结直肠癌肿瘤实质细胞MMP -2的阳性表达率 ( 4 2 .3 % ,5 8例 )高于对照组 ( 15 .0 % ,3例 ) (P <0 .0 5 ) ;发生转移的结直肠癌肿瘤实质细胞其MMP -2阳性表达率明显高于无转移者 (P <0 .0 1) ;低 (未 )分化结直肠癌MMP -2阳性表达率高于高、中分化大肠癌 (P <0 .0 1及P <0 .0 5 )。肿瘤间质中MMP -2阳性表达率 ( 60 .6% ,83例 )明显高于肿瘤交界的正常肠粘膜间质 ( 10 .2 % ,14例 ) (P <0 .0 1)。结论　MMP -2表达与结直肠癌的分化程度及转移密切相关 ,与性别、年龄、肉眼类型、病变部位无明显相关性。Objective To evaluate the expression of Matrix metalloproteinase 2(MMP 2) in colorectal tumor epithelial cells and mesenchymal cells.Methods MMP 2 expression was studied in 137 cases of colorectal carcinoma by immunohistochemistry(LSAB method),12 cases of adenomatous polyp and 8 cases of normal colonic mucosa as control.Results In all 137 colorectal carcinomas,positive staining for MMP 2 in colorectal tumor epithelial cells was obtained in 58(42.3%),and in control group it was 3 of the 20 cases(15.0%)( P <0.05).The positive rate of MMP 2 in metastatic cancer was significantly higher than that in non metastatic cancer( P <0.01).The positive rate of MMP 2 in low or non differentiation carcinoma was higher than that in mid and high differentiation colorectal carcinoma( P <0.01 and P <0.05).The positive expression of MMP 2 within the mesenchymal cells was detecable in 83 of 137 carcinoma,higher than that in non neoplastic mucosa adjacent to tumor( P <0.01).Conclusion The expression of MMP 2 is associated with differentiation and metastasis,and isn′t associated with sex,age,macroscopical pattern and location.

关 键 词：MMP-2 表达 肿瘤实质 肿瘤间质 基质金属蛋白酶-2 大肠癌 

分 类 号：R735.34[医药卫生—肿瘤]