次声作用对大鼠大脑皮层脂质过氧化的影响  

Effects of infrasound on the pallial lipid peroxidation in rats

作　　者：叶琳[1] 龚书明[1] 陈耀明[1] 王枫[1] 刘静[2] 陈景藻[2] 王晶[3] 

机构地区：[1]第四军医大学预防医学系营养与食品卫生学教研室,710032 [2]第四军医大学西京医院康复理疗科 [3]山东省军区综合仓库

出　　处：《中华物理医学与康复杂志》2002年第6期360-362,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：全军医学科学技术研究"十五"计划指令性课题 ( 0 1L0 71)

摘　　要：目的　观察 8Hz、1 30dB次声作用于大鼠不同时间后 ,大脑皮层脂质过氧化相关指标的变化 ,以探讨次声引起脑损伤的机理。方法　将 35只II级、雄性、SD大鼠随机分为对照组和次声作用 7d、1 4d、2 1d、2 8d组五组 ,用 8Hz、1 30dB次声作用 ,每天 1次 ,每次 2h,各组于最后一次作用结束后 0 .5～ 1h内取大脑皮层 ,制成 1 0 %的组织匀浆测定GSH -Px活性、MDA含量和SOD活性的变化。结果　与对照组相比 ,大鼠大脑皮层GSH -Px活性在 7d组无显著性变化 (P >0 .0 5) ,在 1 4d组、2 1d组、2 8d组有显著性升高 (P <0 .0 5) ;MDA含量在 7d组、1 4d组有显著性升高 (P<0 .0 5) ,2 1d组、2 8d组恢复至对照组水平 (P >0 .0 5) ;SOD活性有下降的趋势 ,但无显著性意义 (P >0 .0 5)。结论次声作用后 ,引发大鼠大脑皮层组织的脂质过氧化 抗氧化反应 。Objective To explore the effects of different times of exposure to infrasound on brain tissues in rats. Methods Thirty five male SD rats were equally and randomly divided into 5 groups:a control group, and four infrasound irradiation groups in which the rats were exposed to 8 Hz, 130 dB infrasound once a day and lasted 2 hours each time. Then, the levels of pallial glutathione peroxides(GSH-Px), malondialdehyde(MDA) and superoxide dismutase(SOD) of the irradiated rats were measured 9,14,21,and 28 days, respectively after the exposure. Results In the infrasound irradiation groups, the pallial GSH-Px content was more significantly increased at 14d ( P <0.05) than that in the control group, but there was no obvious difference before 7d ( P >0.05). The content of MDA was also increased more significantly before 14 d in the infrasound groups than that in the control group( P <0.05), but after 21d, it decreased to the level of the control group( P >0.05);The activity of SOD of the infrasound was not changed as compared with the control group( P >0.05). Conclusion Based on the findings, we can make the conclusion that 8 Hz 130 dB infrasound may induce the lipid peroxidation and anti peroxidation that damage the tissues and cells resulting in harmful effect on the brain tissues and cells.

关 键 词：次声作用 大鼠 大脑 皮质 脂质过氧化 丙二醛 

分 类 号：R363[医药卫生—病理学]