DMN肝纤维化模型肝组织I、Ⅲ型胶原和TIMP-1 mRNA表达及复方861的干预作用  

The effects of compound 861 on collagen I?collagen Ⅲ and TIMP-1 mRNA expression in DMN induced liver fibrosis in rats

作　　者：申凤俊[1] 朱跃科[1] 阴宏 毛佳[2] 马雪梅[2] 马红[2] 贾继东[2] 王宝恩[2] 

机构地区：[1]首都医科大学,2000级博士研究生100054 [2]首都医科大学附属北京友谊医院肝病研究中心,100050

出　　处：《临床和实验医学杂志》2002年第3期150-153,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的　研究二甲基亚硝胺 (DMN)肝纤维化模型I型胶原 (CoL -1)、Ⅲ型胶原 (CoL -Ⅲ )和基质金属蛋白酶组织抑制因子 (TIMP -1)mRNA表达以及复方 86 1的干预作用。材料和方法　采用 1%DMN 1ml /kg腹腔注射模型组大鼠 ,复制DMN诱导的大鼠肝纤维化模型 ,同时复方 86 1灌胃 3g/kg ,共 8周 ,以RT -PCR法观察肝纤维化模型肝组织CoL -Ⅰ、CoL -Ⅲ及TIMP -1mRNA表达水平。结果　DMN大鼠肝纤维化模型组CoL -I、CoL -Ⅲ及TIMP-1mRNA分别为 0 82 7± 0 0 94、 0 95 3± 0 0 33及 0 92 4± 0 110 ,明显高于正常对照组 (P <0 0 1) ;复方 86 1治疗组CoL -I、CoL -Ⅲ及TIMP -1mRNA为 0 4 97± 0 0 5 7、 0 85 1± 0 0 6 5和 0 6 48± 0 10 7,明显低于模型对照组 (P <0 0 1或P <0 0 5 )。结论　DMN肝纤维化模型肝组织CoL -Ⅰ、CoL -Ⅲ及TIMP -1mRNA表达水平增高 ,复方 86 1能够抑制肝组织CoL -Ⅰ、CoL -Ⅲ及TIMP -1mRNA的表达 ,从而发挥其抗肝纤维化的作用。Objective To observe the effects of compound 861 (Cpd 861) on collagen I (CoL-I)?collagen Ⅲ(CoLⅢ) and tissue inhibitor of metalloproteinase 1 (TIMP1) mRNA expression in DMN induced liver fibrosis in rats. Methods Excepting rats in control group, all rats were given intraperitoneal injections of 1% dimethylnitrosamine(DMN 1ml/kg? three or two consecutive days/a week for 6 weeks), some of the rats were treated with Cpd 861 in a dose of 3g / kg body weight. All rats were sacrificed at 8 weeks after treatment. Col-I?Col-Ⅲ and TIMP-1 mRNA in the rat livers were detected by RT-PCR. Results Col-I?Col-Ⅲ and TIMP1 mRNA levels of model group were higher than those of control group (P<0.01)Cpd 861 downregulated elevated Col-I ?Col-Ⅲ and TIMP-1 mRNA levels (P<0.05). Conclusion Col-I ?Col-Ⅲ及 TIMP-1 mRNA levels are decreased by Cpd 861 in DMN induced liver fibrosis in rats .

关 键 词：二甲基亚硝胺 肝纤维化 I型胶原 Ⅲ型胶原 基质金属蛋白酶组织抑制因子 

分 类 号：R575.2[医药卫生—消化系统]