增生性玻璃体视网膜病变增生膜内炎性细胞因子m RNA表达  

The m RNA expression of proinflammatory factors in periretinal membrane of proliferative vitreoretinopathy

作　　者：田艳明[1] 惠延年[1] 宋宗明[1] 马吉献[1] 

机构地区：[1]第四军医大学西京医院眼科,西安710032

出　　处：《中华眼底病杂志》2002年第4期286-288,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的　检测炎前因子 m RNA在增生性玻璃体视网膜病变 (proliferative vitreoretinopathy,PVR)中的表达情况 ,证实其在 PVR发病中的作用。　方法　收集 14例不同分级的 PVR患者玻璃体切割术中所取出的增生膜 ,石蜡包埋切片 ,用生物素标记的寡核苷酸探针进行原位杂交 ,检测 IL- 1β、IL- 6、IL- 8和 TNF- α m RNA的表达 ,两例角膜移植后的供体眼作为正常对照。　结果　共有 5例样本有阳性表达。 3例增生膜中 IL- 1βm RNA表达 ,3例表达了 IL- 6 m RNA,1例表达了 IL- 8m RNA,3例表达 TNF- α m RNA。其中 1例既表达了 IL- 1β又表达了 IL- 6 m RNA,1例 IL- 1β、IL- 8和 TNF- α m RNA三者均表达 ,2例有 IL- 6和 TNF- α m RNA两者表达。正常视网膜未检测到炎前因子 m RNA的表达。　结论　IL- 1β、IL- 6、IL- 8和TNF- α参予了 PVR增生膜的形成过程。Objective To observe the expression of proinflammatory factors messenger RNA (mRNA) in periretinal membrane of proliferative vitreoretinopathy. Methods Fourteen specimens of periretinal membrane were collected during vitrectomy, and they were made into paraffin sections.The presence of mRNA coding for IL-1,IL-6,IL-8 and TNF alpha was observed by in situ hybridization(ISH) with biotin labeled oligonuclotide probes respectively.The eyeball after corneal grafting was made as normal control. Results No expression of proinflammatory factors mRNA was found in normal human retina.Positive staining was present in 5 specimens.In these specimens, IL-1β mRNA was found in 3 specimens and TNFα mRNA was found in 3 specimens,there is 1 specimen expressed IL-8 mRNA and 3 specimens expressed IL-6 mRNA.In these positive specimens, one contained cells expressing mRNA for IL-1 β beta and IL-6, and one exhibited cells expressing mRNA for IL-1β?IL-8 and TNF α,two membranes possessed positive cells for IL-6 and TNFα mRNA, one membrane contained IL-1β mRNA positive cells only. Conclusion These findings suggest that these cytokines may be locally produced by cells infiltrating epiretinal membranes. The expression of IL-1β, IL-6, IL-8 and TNFα mRNA within retinal membranes provides further evidence of a pathogenic role of these cytokines in proliferative vitreoretinopathy.

关 键 词：增生性玻璃体视网膜病变 增生膜 炎性细胞因子 表达 原位杂交 PVR 

分 类 号：R774.12[医药卫生—眼科]