p53和bcl-2在老年性白内障晶状体上皮细胞中的蛋白表达  

Protein expression of p53 and bcl-2 in senile cataract lens epithelial cells

作　　者：李元彬[1] 陈薇[2] 朱风云[1] 李守明[1] 杨宪勇[1] 

机构地区：[1]山东省泰安市中心医院眼科,271000 [2]山东大学医学院,250013

出　　处：《眼科新进展》2002年第6期387-389,共3页Recent Advances in Ophthalmology

摘　　要：目的　研究 p5 3、bcl- 2基因在老年性白内障晶状体上皮细胞中的蛋白表达 ,探讨p5 3、bcl- 2在老年性白内障晶状体上皮细胞凋亡中的作用。方法　应用免疫组织化学方法 ,对 30例老年性白内障及 10例正常透明晶状体上皮细胞进行 p5 3、bcl- 2基因蛋白表达的检测 ,计算 p5 3、bcl- 2基因蛋白表达阳性细胞百分率。结果　 p5 3在正常晶状体上皮细胞中无蛋白表达 ,在老年性白内障晶状体上皮细胞中蛋白表达率为 16 .9%～ 19.1% ,bcl- 2在 2组中均无蛋白表达。结论　 p5 3在老年性白内障晶状体上皮细胞中蛋白高表达 ,p5Objective To study the protein expression of p53 and bcl 2 in senile cataract lens epithelial cells,and to assess the relation between p53,bcl 2 and apoptosis of senile cataract lens epithelial cells. Methods With the SABC methods , staining by using DAB,detective the protein expression positive cell rate of p53 and bcl 2. Results In the cells from the normal transparent lenses,almost no p53 and bcl 2 protein expression positive cells,but in the cells from the senile cataract lenses , the rate of positive cells was 16 9%~19.1% , no bcl 2 protein expression positive cells.Conclusion p53 protein expression is higher in senile cataract lens epithelial cells. p53 may be promote apoptosis of the lens epithelial cells from senile cataract.

关 键 词：白内障 晶状体 上皮细胞 蛋白表达 P53 BCL-2 

分 类 号：R776.1[医药卫生—眼科]