两个新型多拷贝毕赤酵母表达载体的构建  

Construction of two new multi-copy Pichia expression vectors

作　　者：齐连权[1] 陈薇[1] 于长明[1] 来大志[1] 翁少洁[1] 王海涛[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《军事医学科学院院刊》2002年第4期264-266,共3页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的 :构建合适的载体用于外源基因在巴斯德毕赤酵母中的正确分泌表达。方法 :利用PCR技术 ,对已有载体pPIC9K进行改造 :在多克隆位点处增加一个NaeⅠ酶切位点 ;去除卡那霉素抗性基因中的一个XhoⅠ酶切位点。结果和结论 :成功地构建了pMEN9K ,pMEX9K两个载体 。Objective:To construct suitable vectors for the secretory expression of heterologous proteins in Pichia pastoris .Methods:A new unique restriction site Nae Ⅰ was added in multiple cloning sites and a restriction site Xho Ⅰ was eliminated in kanamycin resistance gene.Results and Conclusions:Two new vectors pMEN9K and pMEX9K derived from pPIC9K were successfully constructed,and they are suitable for the secretory expression of heterologous proteins.

关 键 词：巴斯德毕赤酵母 载体 基因表达 PCR 

分 类 号：Q782[生物学—分子生物学]