丹参香豆酸-3-羟化酶基因(SmC3H)的生物信息学及其组织表达模式分析  

Bioinformatics and Tissue Expression Pattern Analysis on the Coumarate-3-Hydroxylase Gene(C3H) in Salvia miltiorrhiza Bunge

作　　者：王斌[1] 韩立敏[1] 化文平[1] 陈尘[1] 

机构地区：[1]药用资源与天然药物化学教育部重点实验室,西北濒危药材资源开发国家工程实验室,陕西师范大学,生命科学学院,西安710062

出　　处：《基因组学与应用生物学》2015年第4期813-820,共8页Genomics and Applied Biology

基　　金：江西省教育厅科技项目(编号:GJJ11492)资助

摘　　要：通过分析丹参转录组数据库(SRX021907)得到一条香豆酸-3-羟化酶(C3H)基因,blast比对发现该基因为丹参C3H(EU358957.1),该基因c DNA全长1 704 bp,包括1个1 503 bp的开放阅读框,编码508个氨基酸。生物信息学分析表明,其编码蛋白的分子量为57.21 k D,等电点为8.53,含琢-螺旋,延伸链和无规则卷曲,具有1个细胞色素P450蛋白(CYP450)特异的保守结构域。聚类分析表明其与芝麻的亲缘关系最近。组织特异性表达分析结果表明,该基因在茎中的表达量最丰富,其次是叶和花,根中最低。By analyzing the transcription database(SRX021907) of Salvia miltiorrhiza a gene of coumarate-3-hydroxylase was got. It is named Sm C3 H. The full-length c DNA sequence of Sm C3 H is 1 704 bp, which includes a1 503 bp opening reading frame, encodes a 508-amino-acid protein. Bioinformatics analysis showed that molecular weight of coumarate-3-hydroxylase is 57.21 k D, theory isoelectric point is 8.53, including alpha-helix,extend strand and random coil. It also includes one of distinctive conserved domain database of Cytochrome P450proteins(CYP450). Clusterc analysis revealed that Sm C3 H had high similarity with p-coumarate-3-hydroxylase of Sesamum indicum. Quantitative real-time PCR results revealed the highest expression level of Sm C3 H in stem followed by leaf and flower. The relatively lower expression level in root

关 键 词：丹参 香豆酸-3-羟化酶 生物信息学 组织表达模式 

分 类 号：S567.53[农业科学—中草药栽培]