利用基因芯片对MxIRT1蛋白膜泡在铁胁迫条件下外排途径的研究  

MxIRT1 Protein Vesicle-Trafficking under Iron Deprivation Using Microarrays

作　　者：王俐勇[1] 赵焕英[1] 付文卓[1] 隋雷鸣[1] 

机构地区：[1]首都医科大学医学实验与测试中心,北京100069

出　　处：《基因组学与应用生物学》2015年第5期1047-1052,共6页Genomics and Applied Biology

基　　金：北京市教委科技发展基金重点项目(KZ200710028013)资助

摘　　要：本文以研究缺铁条件下Mx IRT1蛋白膜泡在细胞中的外排途径机制为目的,采用基因芯片方法,发现在铁胁迫环境中,膜泡运输通路相关基因上调表达,推测Mx IRT1蛋白膜泡通过粗面内质网运送至高尔基体,然后通过内体再运送至质膜。该研究有助于进一步研究高等植物中Mx IRT1蛋白相关的铁转运机制。T o c lari fy molecular mechanisms involved in cells' underlying vesicle-trafficking responses to an iron deficiency, Mx IRT1 protein vesicles pathway were examined using global-scale microarrays. Under iron deprivation, most gene about the Mx IRT1-vesicles were up-regulated, and it's speculated that these vesicles were transmitted towards golgi apparatus through rough endoplasmic reticulum(ER) in the absence of iron and transported to plasma membrane via endosomes, which contribute to research of iron transportation about Mx IRT1 protein in higher plants.

关 键 词：猪繁殖与呼吸综合征病毒(PRRSV) 双功能单链抗体 序列分析 蛋白结构预测 

分 类 号：Q945.78[生物学—植物学]