基于denovo测序分析Bt菌株S3299-1基因组特征  

Genome Characterization of Bacillus thuringiensis S3299-1 by De Novo Sequencing

作　　者：黄飞燕[1,2] 李莉[1,2] 周燕 魏艳军[3] 刘盼盼[3] 李有志 张岩[3] 方宣钧[1,2,4] 

机构地区：[1]亚热带农业生物资源保护与利用国家重点实验室广西大学生命科学与技术学院,南宁530004 [2]海南省海德热带农业资源研究所,三亚572025 [3]哈尔滨医科大学生物信息科学与技术学院,哈尔滨150081 [4]浙江农林大学暨阳学院生命科学研究所,诸暨311800

出　　处：《基因组学与应用生物学》2015年第6期1232-1238,共7页Genomics and Applied Biology

基　　金："中国Bt菌收集鉴定计划"项目;广西研究生教育创新计划项目(YCBZ2014006)共同资助

摘　　要：分离菌株S3299-1是从海南省吊罗山热带雨林自然保护区土壤中分离保存的对小菜蛾具有杀虫活性的苏云金芽孢杆菌,但目前尚未明确该菌株的杀虫蛋白基因。本研究主要采用Illumina Hiseq2000二代测序平台对分离菌株S3299-1基因组进行Survey测序以挖掘该菌株的杀虫蛋白基因。研究结果表明:S3299-1基因组的大小为5 498 939 bp,GC含量为34.94%,Scaffold 176个,ORF 5 755个,将Scaffold进行拼接得到4个复制子,即一个大小为4 338 kb的拟核和3个不同的质粒(S3299-1p01,S3299-1p02,S3299-1p03),并预测可能含有的毒蛋白编码基因有25个。通过草图测序以及生物信息学分析手段获得S3299-1的框架图,预测该菌株可能含有的杀虫蛋白基因以及为今后研究该菌株的杀虫蛋白功能提供基础。Bacillus thuringiensis strain S3299-1, was isolated from soil samples, collected from Diaoluoshan Tropical Rainforest Reserve in Hainan province of China, which had been identified with highly larvacidal toxicity against Diamondback Moth(Plutella xylostella). However, it was not yet to know the feature of the insecticidal protein gene of S3299-1. In this work, based on the next generation sequencing of Illumina Hiseq2000 and de-novo assembly, we constructed a high-quality draft genome of S3299-1. The result showed that the genome size of S3299-1 was 5 498 939 bp with 176 scaffolds, 5 755 ORFs and contained four replicons, with a 4 338 kb bacterial chromosome and three plasmids named as S3299-1p01, S3299-1p02 and S3299-1p03, respectively. The average GC content of S3299-1 was 34.94%. Furthermore, we identified 25 potential toxin genes by bioinformatics analysis. With predicted draft genome and the potential toxin genes of this strain, we could do more in depth research in the future.

关 键 词：苏云金芽孢杆菌 de novo测序 基因组 毒蛋白 生物信息学分析 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]