恒定磁场与硫酸肝素对人肝癌细胞系增殖和凋亡的影响  

Study on effects of heparan sulphate and constant magnetic fields on proliferation and apoptosis of human hepatoma cell line(HepG2)

作　　者：王玉苹[1] 张海伟[3] 罗英儒[4] 宋秀豹[1] 叶仲毅 

机构地区：[1]暨南大学医学院第一附属医院康复理疗科,广州510632 [2]暨南大学医学院第一附属医院检验科,广州510632 [3]暨南大学医学院病理教研室,广东省"五个一"兴教实验肿瘤重点实验室 [4]暨南大学医学院药理教研室

出　　处：《中华物理医学与康复杂志》2002年第5期276-278,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：广东省卫生厅及其"五个一"兴教工程基金资助 (粤卫[1998] 2 4 1号A19982 94 )

摘　　要：目的　探讨恒定磁场与硫酸肝素对人肝癌细胞增殖和凋亡的影响及可能机制。方法　体外观察硫酸肝素作用于人肝癌细胞系 (HepG2 )后的细胞增殖、凋亡效应和细胞ras基因表达的变化 ,并探讨其在恒定磁场作用下的相互关系。结果　硫酸肝素能抑制细胞HepG2的增殖能力及其细胞ras基因蛋白的表达 ,并诱导其细胞凋亡率增加。同时 ,在恒定磁场作用下获得细胞增殖抑制与凋亡诱导的加强。结论　硫酸肝素对人肝癌细胞增殖、凋亡的影响与ras基因蛋白介导的信号传递作用有关 ;Objective To explore the effects of heparan sulphate in combination with constant magnetic fields on the proliferation and apoptosis of the human hepatoma cell line (HepG2). Methods Serial experimental observations, including the effects of heparan sulphate on the proliferation and apoptosis of HepG2 as well as the influences of constant magnetic fields on these effects, were carried out in vitro. Results Heparan sulphate could slow-down the progression of HepG2 proliferation (including the ras gene expression level, as well),and these effects were accelerated under the influences of constant magnetic fields. Conclusion The in vitro experimental results suggested that the effect of heparan sulphate on proliferation and apoptosis of HepG2 was correlated with the signalling transduction mediated by ras gene protein, and that a synergic action was present between heparan sulphate and constant magnetic fields on these changes.

关 键 词：恒定磁场 硫酸矸素 肝癌 细胞增殖 RAS蛋白 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]