中国急性弛缓性麻痹病例高发地区脊髓灰质炎病毒分离株性质的研究  

A Study on Molecular Characterization of Poliovirus Isolates from AFP Cases in Zhenxiong County of Zhaotong Prefecture of Yunnan Province in 1997-2001

作　　者：陈立[1] 张礼璧[1] 祝双利[1] 徐闻[2] 朱晖[1] 千叶靖男[3] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100050 [2]云南省疾病预防控制中心,昆明650022 [3]日本国际协力事业团中国消灭脊髓灰质炎项目专家室,北京100050

出　　处：《中国计划免疫》2002年第6期301-305,共5页Chinese Journal of Vaccines and Immunization

摘　　要：中国云南省昭通地区从 1 997年以来每年都是急性弛缓性麻痹 (Acuteflaccidparalysis,AFP)病例的高发地区 ,该地区的镇雄县 1 997～ 2 0 0 1年每年都有≥ 2例由Ⅱ型脊髓灰质炎 (脊灰 )疫苗相关株引起的AFP病例。日本国际协力事业团 (JICA)中国消灭脊灰项目的临床专家曾于 2 0 0 1年赴当地核查这些AFP病例 ,最终临床确认了 4例脊灰 ,认为存在脊灰病例聚集现象。针对此情况 ,对云南省镇雄县 1 997～ 2 0 0 1年从AFP病例中分离到的Ⅱ型脊灰毒株 (共 1 3株 )进行了序列分析 ,发现其VP1和 3D区核苷酸序列变化位点、变化数目及改变各不相同 ,其中有 8株为S2×S3重组株 ,5株为S2×S2疫苗相关株。病毒基因的分析不支持聚集病例间毒株的传播关系 ,不存在单一毒株循环引起的聚集现象。发现其中 1株为脊灰疫苗衍生病毒 (VDPV) ,但未发现其自然循环引发疾病流行。Zhenxiong County of Zhaotong prefecture of Yunnan Province is an acute flaccid paralysis(AFP)case highly occurring area since 1997 There were more than 2 AFP cases per year from 1997～2000 caused by type Ⅱ polio vaccine associated strains Four of the AFP cases examined in 2001 by Japanese expert physicians of JICA were given diagnosis of clinical poliomyelitis The 4 cases were clustered epidemiologically So we sequenced VPI and 3D regions of 13 polio type Ⅱ strains from AFP cases in Zhenxiong County derived from 1997-2001 The sequencing results,however,indicated that the four strains were derived from different viruses,and that the cluster was not due to circulation of a single virus strain Sequencing showed no relationship between any of the 13 type Ⅱ isolates of AFP cases in this one county It suggests that type Ⅱ strains may have many independent lineages but rarely circulate for prolonged periods

关 键 词：中国 急性弛缓性麻痹 脊髓灰质炎病毒 脊髓灰质炎疫苗衍生病毒 脊髓灰质炎疫苗重组株病毒 核苷酸序列分析 AFP 云南 昭通地区 

分 类 号：R512.4[医药卫生—内科学]