螺旋霉素发酵工艺的研究  

Study on the fermentation process of spiramycin

作　　者：王欣荣[1] 刘淑奎[1] 夏永[1] 谢丽芳[1] 

机构地区：[1]四川抗菌素工业研究所,成都610051

出　　处：《中国抗生素杂志》2002年第12期720-721,共2页Chinese Journal of Antibiotics

摘　　要：螺旋霉素产生菌螺旋霉素链霉菌变种 A.sp 99- 4经紫外光照射、光回复突变及螺旋霉素耐受等复合处理 ,使摇瓶发酵效价提高 30 % ;在摇瓶发酵培养基中加入正丙醇及 FH12 ,使发酵效价进一步提高4 0 % ;并初步研究了工艺的中试放大。Streptomyces spiramyceticus A.sp.99 4 was subjected to mutation by compound treatments of mutagenic factors such as ultra violet radiation, photoreactivation and spiramycin tolerance plate test. A strain with 30% higher productivity than the parent strain in the shaking flasks was isolated. The follow up fermentation process with two precursors (n propanol and FH12) was conducted, and the yield was further increased by 40%. Parameters which closely related to the scale up of fermentation process were also discussed.

关 键 词：螺旋霉素 发酵工艺 研究 紫外光处理 光回复突变 

分 类 号：TQ465.5[化学工程—制药化工] R978[医药卫生—药品]