局部注射VEGF挽救大鼠背部随意型超比例皮瓣成活的最佳注射层次的实验性研究  

The Effect of Different Injection Layer of VEGF on Survival of a Random Flap in the Rat.

作　　者：于子莹[1] 张茹慧[1] 刘春丽[1] 朱镇[1] 刘志辉[1] 

机构地区：[1]吉林大学口腔医院,吉林长春130041

出　　处：《口腔医学研究》2002年第6期372-374,共3页Journal of Oral Science Research

摘　　要：目的 :探讨局部注射VEGF挽救大鼠背部随意型超比例皮瓣成活的最佳注射层次。方法 :选用 4 0只Wistar大鼠 ,随机分成四组 ,在其背部形成 2cm× 8cm的全厚随意型皮瓣。术后即刻作如下处理 :在其远蒂端 6cm以及 8cm处选择五个对称的注射位点 ,对照组皮瓣掀起后立即原位缝合 ;VEGF1 组皮瓣掀起后经组织面向皮下每一位点给予 2 0ng 10 0 μl的VEGF溶液 1ml;VEGF2 组皮瓣掀起后立即原位缝合 ,经皮垂直进针 ,将药物注入受区表面 ,剂量同VEGF1 组 ;VEGF3组皮瓣掀起后立即原位缝合 ,经皮垂直进针 ,有落空感后 ,继续垂直进针约 1mm ,将药物注入受区下 ,剂量同VEGF1 组。术后观察 5d后 ,将动物处死 ,计算皮瓣成活面积。结果 :术后第 3d皮瓣成活率 :对照组为6 5 .8% ,VEGF1 组为 83.5 6 % ,VEGF2 组为 75 .79% ,VEGF3组为 83.4 5 %。VEGF2 组与对照组间有显著性差异 (P <0 .0 5 ) ;VEGF1 组、VEGF3组与对照组间有显著性差异 (P <0 .0 1)。结论 :本研究结果提示 :皮瓣内、受区表面、受区下注射VEGF都能显著提高皮瓣的成活率 ;Objective:The puruose of the present study was to determine the effects of different injection layer of vascular endothelial growth factor (VEGF) on survival of a full thickness random pattern,McFarlane musculocutaneous flap in the rats.In addition,this study examined the effects of different dose of VEGF on the flap viability.Methods:A2×8cm full thickness dorsal flap with the pedicle remaining attached at the posterior end was elevated in40 Wistar rats.The rats was randomised into three groups and received the following treatment: Group VEGF1( n =10): subdermal VEGF injection into the flap(200ng/ml) after flap elavation;Group VEGF2 ( n =10): topical injection onto the recipient bed (200ng/ml) after flap elavation;Group VEGF3 ( n =10): subfascial injection into the recipient bed (200ng/ml) after flap elavation.;Group Control( n =10):without any treatment after flap elavation.Results:After five days recovery,the area of flap survival was measured.Mean flap survival in Group Control was (10.52±1.14);Group VEGF1 was (13.39±2.71); Group VEGF2was (12.54±1.13);Group VEGF3 was(13.35±1.46).There was significant difference between Group VEGF123 and the CONTROL group ( P <0.05, P <0.01).Conclusions:These results support the notion that VEGF rescues tissue at risk of hypoxic damage by inducing angiogenesis nomatter subdermal injection,subfascial injection and topical injection onto the recipient bed;But subdermal injection and subfascial injection is better than topical injection on to the recipient bed.

关 键 词：局部注射 最佳注射层次 血管内皮细胞生长因子 随意型皮瓣成活 大鼠 实验研究 

分 类 号：R782[医药卫生—口腔医学]