纳米二氧化硅对莱茵衣藻光合性能的影响  

Effect of Silica Nanoparticles on Photosynthetic Performance of Chlamydomonas reinhardtii

作　　者：胡雨晴 罗学刚[1,2] 张宇 HU Yu-qing;LUO Xue-gang;ZHANG Yu(College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010;Engineering Research Center for Biomass Materials,Ministry of Education,Mianyang,Sichuan 622010)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]生物质材料教育部工程研究中心,四川绵阳622010

出　　处：《安徽农业科学》2025年第1期70-75,79,共7页Journal of Anhui Agricultural Sciences

基　　金：四川省科技计划重点研究项目(20212YFN0025)。

摘　　要：[目的]通过测定纳米二氧化硅(SiO_(2) NPs)处理绿藻后的生长及光合指标变化,揭示SiO_(2) NPs对水生态环境中藻类光合性能的影响。[方法]以莱茵衣藻(Chlamydomonas reinhardtii)为试验材料,从生长、氧化胁迫、叶绿素含量及叶绿素荧光诱导动力学曲线方面研究了SiO_(2) NPs的生物毒性效应。[结果]96 h、25℃高浓度(800 mg/L)的SiO_(2) NPs诱导莱茵衣藻活性氧(ROS)上升了21.57%,并且抑制了莱茵衣藻光合作用,导致叶绿素a含量下降了10.87%,光合性能指数Pi-Abs下降了50.72%。而温度升高加剧了纳米二氧化硅的毒性。当温度上升至30℃时,高浓度(800 mg/L)的SiO_(2) NPs导致莱茵衣藻ROS比对照组上升了26.58%,叶绿素a和叶绿素b含量分别下降了23.47%和20.35%,光合性能指数Pi-Abs显著下降了55.72%。高浓度SiO_(2) NPs对莱茵衣藻光合作用有一定的毒害作用,且温度升高与SiO_(2) NPs浓度增加的协同作用将加剧莱茵衣藻光合毒性效应。[结论]该研究为探索纳米粉体材料对微藻的光合效应提供了理论基础和数据支持。[Objective]To reveal the effect of SiO_(2) NPs on algal photosynthesis in aquatic ecosystems by determining the changes in growth and photosynthetic indexes of green algae treated with silica nanoparticles(SiO_(2) NPs).[Method]The biotoxic effects of SiO_(2) NPs were studied in terms of growth,oxidative stress,chlorophyll content and chlorophyll fluorescence-induced kinetic curves using Chlamydomonas reinhardtii as experimental material.[Result]At 96 h and 25℃,the high concentration of 800 mg/L SiO_(2) NPs induced a 21.57%increase in reactive oxygen species(ROS)of Chlamydomonas reinhardtii and inhibited the photosynthesis of Chlamydomonas reinhardtii,which resulted in a 10.87%decrease in chl-a content and a 50.72%decrease in the photosynthetic performance index Pi-Abs.And the toxicity of silica nanoparticles was exacerbated by the temperature increase.When the temperature increased to 30℃,the high concentration of 800 mg/L of SiO_(2) NPs led to the increase of reactive oxygen species(ROS)of Chlamydomonas reinhardtii by 26.58%,the decrease of chlorophyll a and chlorophyll b content by 23.47%and 20.35%,and the significant decrease of the photosynthetic performance index Pi-Abs by 55.72%compared with that of the control group.High concentration of SiO_(2) NPs had a toxic effect on photosynthesis of Chlamydomonas reinhardtii,and the synergistic effect of increasing temperature and increasing concentration of SiO_(2) NPs will exacerbate the photosynthetic toxic effect of Chlamydomonas reinhardtii.[Conclusion]This study provides a theoretical basis and data support for exploring the photosynthetic effects of nanopowder materials on microalgae.

关 键 词：纳米二氧化硅 莱茵衣藻 光合效应 叶绿素荧光诱导动力学曲线 活性氧 

分 类 号：X171[环境科学与工程—环境科学]