月见草籽提取物对枯草芽孢杆菌的抑菌活性及其机制研究  

Inhibitory activity and mechanism of the extract of Oenothera biennis L.seeds against Bacillus subtilis

作　　者：宗雪晗 姜雯 张鹏宇 姜春宇 金峰伟 周昊奇 廉美兰[1] 朴炫春[1] ZONG Xuehan;JIANG Wen;ZHANG Pengyu;JIANG Chunyu;JIN Fengwei;ZHOU Haoqi;LIAN Meian;PIAO Xuanchun(Agricultural College of Yanbian University,Yanji Jilin 133002,China)

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《延边大学农学学报》2024年第4期1-8,共8页Journal of Agricultural Science Yanbian University

基　　金：国家自然科学基金(81960685)。

摘　　要：为探明月见草(Oenothera biennisL.)籽提取物对枯草芽孢杆菌的抑菌效果,该研究以月见草籽乙醇提取物(Oenothera biennis L:seeds,OBSD)为材料,探究其对枯草芽孢杆菌的抑菌能力和对细菌细胞膜与呼吸代谢的影响。结果表明:最低抑菌浓度为4mg/mL;经OBSD处理后,细菌细胞膜通透性增大,电导率增大,碱性磷酸酶含量上升,胞内核酸和蛋白质泄漏,扫描电镜显示细菌细胞形态及结构发生异常变化,细胞内容物泄漏,并且OBSD通过抑制糖酵解途径(Embden-meyerhof-parnas,EMP)中的磷酸果糖激酶(Phosphofructo kinase,PFK)、己糖激酶(Hexokinase,HK)及丙酮酸激酶(Pyruvate kinase,PK)的活性抑制枯草芽孢杆菌的呼吸作用,最终起到抑菌效果。该研究探明了月见草籽抑制枯草芽孢杆菌的机制,为月见草籽的使用及开发奠定了基础。In order to investigate the bacteriostatic effect of Oenothera biennis L.seeds extract on Bacillus subtilis,the ethanol extract of O.biennis seeds(OBSD)was used as a material in the present study to investigate its bacteriostatic ability on B.subtilis,as well as its effect on bacterial cell membrane and respiratory metabolism.The results showed that the lowest inhibitory concentration was 4 mg/mL,the permeability of the cell membrane of B.subtilis increased,the conductivity increased,the content of alkaline phosphatase increased,the intracellular nucleic acids and proteins leaked,and the scanning electron microscope showed that the morphology and structure of B.subtilis cells were changed abnormally after treatment with ethanolic extracts of O.biennis seeds.The results of the inves-tigation on the oxidative respiratory metabolism of the bacteria showed that OBSD inhibited the respiration of B.subtilis by inhibiting the activities of phosphofructokinase(PFK),hexokinase(HK)and pyruvate kinase(PK)in the enzymatic pathway(EMP),and finally exerted a bacteriostatic effect.This study elucidated the mechanism of inhibition of B.subtilis by O.biennis seeds and lays the founda-tion for the use and development of O.biennis seeds.

关 键 词：提取物 月见草籽 枯草芽孢杆菌 抑菌活性 呼吸代谢 

分 类 号：R285[医药卫生—中药学]