血清微小RNA-375和微小RNA-760联合肝脏增强CT技术对肝细胞癌的诊断效能  

Diagnostic efficacy of serum microRNA-375 and microRNA-760 combined with liver enhancement computed tomography in hepatocellular carcinoma

作　　者：刘梦雯 柳群力 杨洁 LIU Mengwen;LIU Qunli;YANG Jie(Department of Medical Imaging,the First Affiliated Hospital of Henan University,Kaifeng,Henan,475002)

机构地区：[1]河南大学第一附属医院医学影像科,河南开封475002

出　　处：《实用临床医药杂志》2024年第23期47-51,共5页Journal of Clinical Medicine in Practice

基　　金：2022年度河南省开封市科技发展计划项目(2203032)。

摘　　要：目的探讨血清微小RNA-375(miR-375)、微小RNA-760(miR-760)联合肝脏增强CT技术对肝细胞癌(HCC)的诊断效能。方法选取144例HCC患者纳入癌症组,另选取同期144例非肿瘤肝病患者纳入良性组,检测2组患者血清miR-375、miR-760表达水平。绘制受试者工作特征(ROC)曲线,分析血清miR-375、miR-760表达水平联合肝脏增强CT对HCC的诊断价值。采用Kappa检验分析血清miR-375、miR-760表达水平和肝脏增强CT单独及联合诊断HCC与“金标准”病理结果的一致性。结果癌症组患者血清miR-375、miR-760表达水平均低于良性组,差异有统计学意义(P<0.05)。HCC患者血清miR-375、miR-760表达水平均与肿瘤数目、肿瘤直径、TNM分期、远处器官转移、分化程度、门静脉癌栓、乙型肝炎表面抗原相关(P<0.05)。ROC曲线分析结果显示,血清miR-375、miR-760和肝脏增强CT联合诊断HCC的曲线下面积(AUC)显著大于三者单独诊断的AUC(P<0.05),与病理结果的一致性极高(Kappa=0.826)。结论HCC患者血清miR-375、miR-760表达水平低于非肿瘤肝病患者,血清miR-375、miR-760水平和肝脏增强CT技术三者联用可显著提升对HCC的诊断效能。Objective To investigate the diagnostic efficacy of serum microRNA-375(miR-375)and microRNA-760(miR-760)combined liver enhancement computed tomography(CT)in hepatocellular carcinoma(HCC).Methods A total of 144 patients with HCC were included in cancer group,and another 144 patients with benign liver tumors in the same period were included in benign group.The expression levels of serum miR-375 and miR-760 were measured in both groups.Receiver operating characteristic(ROC)curves were plotted to analyze the diagnostic value of serum miR-375 and miR-760 expression levels combined with liver enhancement CT in HCC.The Kappa test was used to analyze the consistency of lonely detection of serum miR-375,miR-760 levels and liver enhancement CT,and their combined diagnosis for HCC with the gold standard pathological results.Results The expression levels of serum miR-375 and miR-760 in the cancer group were significantly lower than those in the benign group(P<0.05).The expression levels of serum miR-375 and miR-760 in patients with HCC were correlated with the number of tumor,tumor diameter,TNM stage,distant organ metastasis,degree of differentiation,portal vein tumor thrombus,and hepatitis B surface antigen(P<0.05).The ROC curve analysis showed that the area under the curve(AUC)of combined diagnosis of HCC using serum miR-375,miR-760,and liver enhancement CT was significantly greater than the AUC of individual diagnosis of the three methods(P<0.05),and there was a high consistency with pathological results(Kappa=0.826).Conclusion The expression levels of serum miR-375 and miR-760 in patients with HCC are lower than those in patients with benign liver tumors.The combined use of serum miR-375 and miR-760 levels and liver enhancement CT technology can significantly improve the diagnostic efficacy of HCC.

关 键 词：肝细胞癌 微小RNA-375 微小RNA-760 肝脏增强CT 诊断效能 

分 类 号：R735.7[医药卫生—肿瘤] R445[医药卫生—临床医学] R446