磷限制对赫氏艾密里藻光合生理的影响  

Effect of Phosphate Limitation on Photosynthetic Physiology of the Coccolithophore Emiliania huxleyi

作　　者：王茵蕊 李政科 尚金龙 马帅[1] 杨祥 张泳 韩永和[1] 张虹[1] 徐魁 张勇[1] WANG Yinrui;LI Zhengke;SHANG Jinlong;MA Shuai;YANG Xiang;ZHANG Yong;HAN Yonghe;ZHANG Hong;XU Kui;ZHANG Yong(College of Environmental and Resource Sciences,College of Carbon Neutral Modem Industry,Fujian Normal University,Fuzhou 350117,China;School of Food Science and Engineering,Shaanxi University of Science and Technology,Xi an 710021,China;School of Life Sciences,Xinjiang Normal University,Urumqi 830054,China;College of Life Sciences,Hubei Normal University,Huangshi 435002,China)

机构地区：[1]福建师范大学环境与资源学院/碳中和现代产业学院,福建福州350117 [2]陕西科技大学食品科学与工程学院,陕西西安710021 [3]新疆师范大学生命科学学院,新疆乌鲁木齐830054 [4]湖北师范大学生命科学学院,湖北黄石435002

出　　处：《福建师范大学学报(自然科学版)》2025年第1期109-115,共7页Journal of Fujian Normal University:Natural Science Edition

基　　金：国家自然科学基金青年项目(41806129);福建省自然科学基金面上项目(2023J01290)。

摘　　要：颗石藻作为一种主要的钙化浮游植物类群,在海洋碳循环中发挥关键作用,其生长和光合作用受营养盐浓度变化的影响。分别在高浓度磷酸盐条件下细胞生长周期的指数期、低浓度磷酸盐条件下细胞生长周期的稳定期和不添加磷酸盐条件下细胞生长周期的衰亡期,测定颗石藻纲的模式种赫氏艾密里藻的细胞浓度、色素含量和光合活性。结果显示:藻细胞的生长速率、叶绿素含量、光系统Ⅱ的光化学效率和捕光截面随磷酸盐浓度的减少而下降;电子从原初电子受体醌传递到质体醌库的时间随磷酸盐浓度的减少而增加。但在磷酸盐限制条件下培养的藻细胞转接到高浓度磷酸盐条件下,培养4 d后,细胞的生长、色素和光合活性获得很好恢复。结果表明:赫氏艾密里藻能快速响应磷酸盐浓度的波动,在不同生长时期调节生长和光合作用,这是赫氏艾密里藻在磷限制环境中占据优势的重要原因之一。Coccolithophores are one of the most important calcified phytoplankton,playing a key role in the marine carbon cycle.The growth and photosynthesis of coccolithophores are affected by nutrient availability.Cell concentration,cellular pigment content,and photosynthetic activity of Emiliani huxleyi,a model coccolithophore species,were investigated during the exponential growth phase under high phosphate concentration,the stationary growth phase under low phosphate concentration,and the decline growth phase under phosphate-free conditions.The results show that the growth rate,cellular chlorophyll a content,photochemical efficiency,and the functional absorption cross-section of photosystemⅡdecreased with decreasing phosphate availability.The average time for electron transport from the initial electron acceptor to the plastoquinone pool increased with decreasing phosphate availability.When cells under phosphate-free conditions were inoculated into high phosphate concentration,and cultured for 4 days,growth rate,cellular pigment content,and photosynthetic activity of E.huxleyi were restored to levels close to those under high phosphate conditions.These results indicate that E.huxleyi cells can rapidly respond to fluctuations in phosphate availability and adjust their growth and photosynthetic function,which is one of the reasons for E.huxleyi s competitive advantages in phosphate-limited environments.

关 键 词：光合作用 赫氏艾密里藻 颗石藻 磷酸盐 生长 色素 

分 类 号：Q945[生物学—植物学]