老年急性缺血性脑卒中患者血清miR-99a-5p与神经功能缺损程度及炎性因子的相关性  

The correlation between serum mi R-99a-5p and degree of neurological impairment and inflammatory factors in elderly patients with acute ischemic stroke

作　　者：高靖 崔萍萍 王霆[1] 季帅 Gao Jing;Cui Pingping;Wang Ting;Ji Shuai(Department of Neurology,Jiyuan People's Hospital,Jiyuan 459000)

机构地区：[1]济源市人民医院神经内科,河南济源459000

出　　处：《中国现代医药杂志》2024年第12期11-15,共5页Modern Medicine Journal of China

摘　　要：目的 探讨老年急性缺血性脑卒中(Acute ischemic stroke,AIS)患者血清miR-99a-5p与神经功能缺损程度及炎性因子[白细胞介素-6(IL-6)、白细胞介素-8(IL-8)及肿瘤坏死因子-α(TNF-α)]的相关性。方法 采用美国国立卫生研究院卒中量表(NIHSS)将165例老年AIS患者分为轻度组(NIHSS评分<5分)、中度组(NIHSS评分5~20分)及重度组(NIHSS评分>20分),另选择无严重疾病、无器官损害,体检结果正常者60例为对照组。采用PCR技术检测血清miR-99a-5p表达水平,酶联免疫吸附法检测血清IL-6、IL-8及TNF-α水平。应用ROC曲线分析miR-99a-5p对老年AIS的诊断价值,采用Pearson进行相关性分析。结果 AIS组血清miR-99a-5p、IL-6、IL-8及TNF-α水平明显高于对照组(P<0.05)。重度组血清miR-99a-5p、IL-6、IL-8及TNF-α水平均明显高于中度组和轻度组(P<0.05)。血清miR-99a-5p表达水平=1.66时诊断老年AIS的AUC为0.832,敏感度和特异度分别为86.2%和79.4%。相关性分析结果显示,老年AIS患者血清miR-99a-5p表达水平与IL-6(r=0.608)、IL-8(r=0.426)、TNF-α(r=0.518)水平及NIHSS评分(r=0.774)均呈正相关(P<0.05)。结论 血清miR-99a-5p与神经功能缺损程度及炎性因子有关,对老年AIS的诊断具有一定价值。Objective To investigate the correlation between serum miR-99a-5p and the degree of neurological impairment and inflammatory factors such as interleukin-6(IL-6), interleukin-8(IL-8) and tumor necrosis factor-α(TNF-α) in elderly patients with acute ischemic stroke(AIS). Methods 165 elderly patients with AIS were divided into the mild group(NIHSS score<5), the moderate group(NIHSS score 5~20), and the severe group(NIHSS score>20) by National Institutes of Health Stroke Scale(NIHSS). 60 objects without severe diseases, organ damage and normal physical check-up results were selected as the control group. The expression level of serum miR-99a-5p was detected by PCR. The levels of serum IL-6, IL-8 and TNF-α were detected by enzyme-linked immunosorbent assay. The value of miR-99a-5p in the diagnosis of elderly AIS was analyzed using ROC curve analysis, and Pearson was used for correlation analysis. Results The levels of serum miR-99a-5p, IL-6, IL-8 and TNF-α in AIS group were significantly higher than those in the control group(P<0.05). The levels of serum miR-99a-5p, IL-6, IL-8 and TNF-α in the severe group were significantly higher than those in the moderate group and the mild group(P<0.05).The AUC of the serum miR-99a-5p expression level=1.66 was 0.832 for diagnosing elderly AIS, with sensitivity and specificity of 86.2% and 79.4%, respectively. Related analysis showed that the expression level of serum miR-99a-5p in elderly AIS patients was positively correlated with IL-6(r=0.608), IL-8(r=0.426), TNF-α(r=0.518) and NIHSS score(r=0.774). Conclusion Serum miR-99a-5p is related to the degree neurological impairment and inflammatory factors, and has certain value for the diagnosis of elderly AIS.

关 键 词：急性缺血性脑卒中 miR-99a-5p 神经功能缺损程度 白细胞介素-6 白细胞介素-8 肿瘤坏死因子-α 

分 类 号：R74[医药卫生—神经病学与精神病学]