茶皂素的纯化及其对乙醇脱氢酶活性组分分离的影响  

Purification of crude tea saponin and its effect on separation of active components related with alcohol dehydrogenase

作　　者：易醒[1] 李佳奕 周志宇 肖小年[2] YI Xing;LI Jiayi;ZHOU Zhiyu;XIAO Xiaonian(Sino-German Food Engineering Center,Nanchang University,Nanchang 330047,China;Sino-German Joint Research Institute,Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学中德食品工程中心,江西南昌330047 [2]南昌大学中德联合研究院,江西南昌330047

出　　处：《南昌大学学报(理科版)》2024年第6期559-566,573,共9页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(31960455,32360575);农产品质量安全江西省重点实验室(2024SSY04192)。

摘　　要：分别采用正丁醇萃取法和大孔树脂吸附法对粗茶皂素进行纯化处理研究后,探讨了纯化处理对磁性固定化酶分离茶皂素中乙醇脱氢酶活性组分(AAC)的影响。结果显示,纯化后样品中多糖和多酚类物质有所减少,茶皂素的含量明显提高,其中萃取法和大孔树脂吸附法纯化样品中茶皂素含量分别较粗茶皂素提高19.8%和18.5%。将纯化样品应用于AAC的磁性固定化酶分离时,固定化酶的酶活力损失率可以得到有效降低,其中以大孔树脂吸附法的损失率最低,可达27.8%。纯化前后获得的AAC的光谱学特征没有改变,主要仍为茶皂苷类物质。AAC对乙醇脱氢酶的作用未明显改变,均有抑制作用且抑制效果呈浓度依赖性。因此,对粗茶皂素进行纯化处理可降低磁性固定化酶分离过程中酶的用量,提高固定化酶的重复利用性,而对AAC的影响不明显。After the purification of crude tea saponin through the method of n-butyl alcohol extraction or macroporous resin adsorption,the impact of purification on the separation of alcohol dehydrogenase related active components(AAC)from purified tea saponin by magnetic immobilization enzyme was investigated.The results demonstrated a reduction in polysaccharides and polyphenols in the purified samples,while significantly increasing the content of tea saponin.Specifically,the content of tea saponin in the purified samples obtained through extraction method and macroporous resin adsorption method was 19.8%and 18.5%higher than that of crude tea saponin,respectively.When these purified samples were utilized for the separation by magnetic immobilization,it effectively minimized enzyme activity loss ratio associated with immobilized enzymes.Notably,macroporous resin adsorption exhibited the lowest loss ratio at 27.8%.Spectral characteristics analysis revealed no changes in AAC obtained before and after purification;they remained predominantly composed of tea saponins.Furthermore,there were no significant alterations observed in AAC's inhibitory effect on alcohol dehydrogenase;this inhibitory effect remained concentration-dependent as well.Therefore,purifying crude tea saponin can reduce enzyme consumption during magnetic immobilized enzyme separation process while enhancing reusability without significantly affecting AAC.

关 键 词：茶皂素 纯化 活性组分 乙醇脱氢酶 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]