不同类型基质对附着细菌定殖演替和群落结构的影响  

Effects of Different Substrates on Colonization Succession and Community Structure of Adherent Bacteria

作　　者：黄颖婷 范薇[1] 赵恒[1] 朱利明 刘一休[1] 郑志伟[2] 史方[2] 邹曦[2] 王开诚 朱稳 何姗 HUANG Yingting;FAN Wei;ZHAO Heng;ZHU Liming;LIU Yixiu;ZHENG Zhiwei;SHI Fang;ZOU Xi;WANG Kaicheng;ZHU Wen;HE Shan(China Railway Siyuan Survey and Design Group Co.Ltd.,Wuhan 430063,P.R.China;Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources,Institute of Hydroecology,Ministry of Water Resources&Chinese Academy of Sciences,Wuhan 430079,P.R.China)

机构地区：[1]中铁第四勘察设计院集团有限公司,湖北武汉430063 [2]水利部中国科学院水工程生态研究所,水利部水工程生态效应与生态修复重点实验室,湖北武汉430079

出　　处：《水生态学杂志》2025年第1期122-132,共11页Journal of Hydroecology

基　　金：武汉市知识创新专项基础研究项目(2022020801010171,2023020201020299);中铁第四勘察设计院集团有限公司科研课题项目(KY2022051S)。

摘　　要：探讨不同类型基质对附着细菌定殖演替规律和群落结构特征的影响,为利用周丛生物技术开展水生态修复提供参考。通过原位实验,探究5种基质表面附着细菌的定殖演替规律和群落结构特征。结果表明:变形菌门(Proteobacteria)、放线菌门(Actinobacteriota)、拟杆菌门(Bacteroidota)、厚壁菌门(Firmicutes)和蓝细菌门(Cyano-bacteria)是各基质表面附着细菌的主要组成类群,变形菌门在各基质表面都是最先定殖,蓝细菌门和拟杆菌门一般在8 d后才会逐渐成为优势类群,厚壁菌门一般在16 d后才成为优势类群;PCoA结果显示,实验前中期(4~16 d)各基质表面附着细菌群落结构差异显著,实验结束时(第20天)各基质表面附着细菌群落结构趋同,虽有差异但不显著,基质材料物化特性对附着细菌群落结构影响主要在细菌早期发育或定殖阶段;网络结构分析和FA-PROTAX功能预测结果表明,有机质类基质(竹子和木头)表面附着细菌群落共发生网络的复杂性、稳定性、传输性能和效率更高,网络节点间的关系更加密切;其细菌群落功能中的化能异养型和好氧化能异养型功能占比最高,消化分解水中有机物的能力更强。后期开展水生态修复时,应考虑多种类型基质相结合的模式。Adherent bacteria function as decomposers in ecosystems and have become an important component in the ecological restoration of water bodies.Selecting appropriate attachment substrates to enhance synergistic effects among different adherent bacteria and strengthen their ecological restora-tion function is a key research direction.In this study,five substrates(hollow bricks,pebbles,volca-nic rock,bamboo and wood)were selected for comparison.Using in situ experiments conducted in the field,we studied the effects of each substrate on colonization succession and the community struc-ture of adherent bacteria.Our aim was to provide insight and information for selecting substrates that function effectively for water pollution treatment using periphyton biotechnology.The experiment was conducted in an artificial lake from March 4 to March 24,2024.The five substrates,15 of each type,were suspended at a depth of 1m and on days 4,8,12,16 and 20,three of each substrate type was randomly removed for examination of adherent bacteria,and water samples were concurrently collect-ed.Proteobacteria,Actinobacteria,Bacteroidetes,Firmicutes and Cyanobacteria were the primary bac-terial groups attached to the surface of all substrate types.Substrate surfaces were first colonized by Proteobacteria,Cyanobacteria and Bacteroidetes became dominant after 8 days,and Firmicutes gener-ally dominated after 16 days.Principal Coordinates Analysis(PCoA)showed that the bacterial com-munity structures were significantly different among the five substrates at the middle stage(4-16 d),but tended to converge to similar structures by the end of the experiment(20 d).This result indicates that differences in the physicochemical properties of the different substrates primarily affected early-stage bacteria development and colonization,but had little obvious effect on the bacterial community after maturation.However,analysis of network structure and Functional Annotation of Procaryotic Taxa(FAPROTAX)predictions showed that the co-occurrence network of the b

关 键 词：附着细菌 附着基质 定殖演替 共发生网络 群落结构 

分 类 号：X172[环境科学与工程—环境科学]