Tanyu Tongzhi formula attenuates atherosclerotic plaque vulnerability by inhibiting CD40^(+) monocyte differentiation  

作　　者：Zheng-Tian Lv Yan-Yun Pan Chen-Xia Wu Yuan-Gang Qiu Shen-Jie Chen Wei Mao Jin Dai 

机构地区：[1]Department of Cardiology,The First Affiliated Hospital of Zhejiang Chinese Medical University(Zhejiang Provincial Hospital of Chinese Medicine),Hangzhou 310003,China [2]Zhejiang Key Laboratory of Integrative Chinese and Western Medicine for Diagnosis and Treatment of Circulatory Diseases,Hangzhou 310003,China [3]Department of Cardiology,Affiliated Zhejiang Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China

出　　处：《Traditional Medicine Research》2025年第4期61-71,共11页TMR传统医学研究

基　　金：Natural Science Foundation of Zhejiang Province(grant number LY20H290005);Key Research and Development Program of Zhejiang Province(grant number 2020C03119);National Natural Science Foundation of China(grant numbers 81673706,81973579,82174150);Science and Technology Program of Zhejiang Traditional Chinese Medicine(grant number 2018ZY005);TCM science and Technology Foundation Project of Zhejiang Province(grant number 2023ZL411).

摘　　要：Background:Atherosclerosis(AS)is a chronic progressive inflammatory disease,and plaque stability plays a critical role in preventing acute events.Tanyu Tongzhi formula(TTF),a traditional Chinese medicine,has potential therapeutic effects on AS.Methods:We used an AS animal model to examine the effects of TTF on atherosclerotic plaque vulnerability and CD40^(+)monocyte differentiation.AS plaque area,collagen content,and lipid area were assessed using histological staining.AS plaque-related biomarkers(monocyte chemoattractant protein 1(MCP-1),monocyte plus macrophage(MOMA-2),von Willebrand factor(vWF),CD31,and alpha-smooth muscle actin(α-SMA))were detected using immunohistochemistry.Inflammatory cytokines were determined using enzyme-linked immunosorbent assay.CD40 mRNA expression was detected by real-time quantitative PCR.CD40^(+)monocyte differentiation was evaluated by flow cytometry analysis in ApoE-/-mice and peripheral blood mononuclear cells(PBMCs).AMPK/NF-κB signaling pathways-related protein expression was investigated through western blotting.Results:TTF treatment reduced AS plaque area,collagen content,and lipid area in AS model mice.Levels of MCP-1,MOMA-2,vWF,and CD31 were decreased,whileα-SMA level was increased by TTF in model mice.TTF reduced inflammatory cytokines levels,including tumor necrosis factor receptor-α(TNF-α),high-sensitivity C-reactive protein(hs-CRP),and interleukin-6(IL-6).TTF inhibited CD40^(+)monocyte differentiation and decreased the number of CD40^(+)/CD40-and Ly6C^(+)/Ly6C-cells and M1/M2 ratio in AS model mice and human PBMCs.Additionally,TTF modulated the AMPK/NF-κB signaling pathway in human PBMCs.Conclusion:TTF attenuates atherosclerotic plaque vulnerability by inhibiting CD40^(+)monocyte differentiation,possibly through the regulation of the AMPK/NF-κB signaling pathway.These findings suggest that TTF could be a potential therapeutic agent for preventing plaque rupture and subsequent cardiovascular events in patients with AS.

关 键 词：ATHEROSCLEROSIS Tanyu Tongzhi formula CD40^(+)monocyte differentiation inflammation 

分 类 号：R285.5[医药卫生—中药学]