菊苣叶内生真菌的分离及其抗植物病原菌活性分析  

Isolation of endophytic fungi from the leaves of Cichorium intybus and analysis on their antifungal activity against phytopathogens

作　　者：刘洋 孟秀花 吕寒[1] 卢昊 张志伟 牛冠婷 任冰如[1] 陈剑[1] LIU Yang;MENG Xiuhua;LYU Han;LU Hao;ZHANG Zhiwei;NIU Guanting;REN Bingru;CHEN Jian(Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen),Nanjing 210014,China)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园)江苏省植物资源研究与利用重点实验室,江苏南京210014

出　　处：《植物资源与环境学报》2025年第1期118-121,共4页Journal of Plant Resources and Environment

基　　金：国家自然科学基金项目(81973463,32000279)。

摘　　要：采用组织分离法从菊苣(Cichorium intybus Linn.)叶分离内生真菌,结合平板对峙法筛选对9种常见植物病原菌具有拮抗活性的内生真菌,联合形态学和分子生物学进行鉴定,并检测活性内生真菌发酵产物的抑菌效果。结果显示:从菊苣叶中分离得到24株内生真菌,筛选到1株对9种植物病原菌均有较好拮抗活性的内生真菌菌株CIY4,鉴定其为链格孢属(Alternaria Nees)的损毁链格孢(Alternaria destruens)。500μg·mL^(-1)的菌株CIY4发酵产物对水稻纹枯病菌(Rhizoctonia solani J.G.Kühn)的抑制率达到86.31%,其后依次为油菜菌核病菌(Sclerotinia sclerotiorum Lib.)(85.45%)、枸杞炭疽病菌(Colletotrichum acutatum J.H.Simmonds)(77.87%)、橡胶树炭疽病菌(Colletotrichum gloeosporioides Penz.)(71.12%),对其他植物病原菌的抑制率低于70%。本研究首次分离筛选了菊苣叶中内生真菌菌株CIY4,该链格孢属真菌具有普遍抑制常见植物病原菌的作用。Endophytic fungi were isolated from the leaves of Cichorium intybus Linn.by using the tissue separation method,endophytic fungi with antagonistic activity against nine common phytopathogens were screened by using the plate confrontation method,the endophytic fungi were identified by using a combination of morphology and molecular biology,and the antifungal effects of the fermentation products of the active endophytic fungi were assayed.The results show that a total of 24 endophytic fungi are isolated from the leaves of C.intybus,one endophytic fungal isolate CIY4 that shows good antagonistic activity against all nine phytopathogens is screened out,and it is identified as Alternaria destruens belonging to the genus Alternaria Nees.The inhibition rate of 500μg·mL-1 fermentation products of isolate CIY4 against Rhizoctonia solani J.G.Kühn reaches 86.31%,followed by Sclerotinia sclerotiorum Lib.(85.45%),Colletotrichum acutatum J.H.Simmonds(77.87%),and Colletotrichum gloeosporioides Penz.(71.12%),while the inhibition rates against the other phytopathogens are lower than 70%.This study isolates and screens the endophytic fungal isolate CIY4 from the leaves of C.intybus for the first time,and this Alternaria fungus exhibits a broad-spectrum inhibitory effect against common phytopathogens.

关 键 词：菊苣 叶 内生真菌 植物病原菌 抗菌活性 

分 类 号：Q946.96[生物学—植物学] S482.292[农业科学—农药学]