筇竹钾离子通道QtSKOR2基因的克隆及表达分析  

Cloning and Expression Analysis of QtSKOR2 Gene of Qiongzhuea tumidinoda Potassium Ion Channel

作　　者：王玥琪 赵小艳 芮蕊[1] Wang Yueqi;Zhao Xiaoyan;Rui Rui(Yunnan Key Laboratory of Underforest Resource Protection and Utilization,Southwest Forestry University,Kunming,650224,China)

机构地区：[1]西南林业大学/云南省林下资源保护与利用重点实验室,昆明650224

出　　处：《世界竹藤通讯》2024年第6期18-26,共9页World Bamboo and Rattan

基　　金：云南省万人计划青年拔尖人才项目(YNWR-QNBJ-2019-028);国家现代农业产业技术体系建设专项(CARS-21);云南省重大科技专项(202102AE090042,2019ZG00901);昆明市科技揭榜制项目(2021JH002)。

摘　　要：为了研究SKOR家族在筇竹(Qiongzhuea tumidinoda)钾离子运输中发挥的作用,利用克隆技术从筇竹的幼苗中克隆出SKOR基因并命名为QtSKOR2,随后对其理化性质和表达模式进行分析。结果显示,QtSKOR2基因全长为2334 bp,开放阅读框为2139 bp,编码712个氨基酸。同时,QtSKOR2蛋白有6个跨膜结构域,且与芦苇(Phragmites australis)的同源相似性最高,为90.46%。聚类分析表明,QtSKOR2蛋白与短花稻(Oryza brachyantha)的亲缘关系最近。QtSKOR2基因在筇竹所有组织中均有表达,且表达量依次为根>笋>叶>茎。在50 mmol/L KCl胁迫处理下,QtSKOR2基因在筇竹根部表达量最高,且表达量随着时间的延长而增加。而在250 mmol/L NaCl胁迫处理下,QtSKOR2基因在筇竹叶片中表达量最高,且表达量随着时间的延长而增加。本研究结果揭示了盐胁迫下QtSKOR2基因在筇竹钾离子运输中可能发挥着重要作用。To investigate the function of the SKOR family in potassium ion transport in Qiongzhuea tumidinoda,a gene named QtSKOR2 is cloned from the young seedlings of Q.tumidinoda using cloning technology,and its physicochemical properties and expression pattern are analyzed.The result shows that the full length of the QtSKOR2 gene is 2334 bp,with an open reading frame of 2139 bp,which encodes 712 amino acids.Moreover,the QtSKOR2 protein has six transmembrane domains and exhibited the highest homology similarity with that of Phragmites australis at 90.46%.Cluster analysis suggests that the QtSKOR2 protein is most closely related to that of Oryza brachyantha.Additionally,the QtSKOR2 gene is expressed in all tissues of Q.tumidinoda,with the highest expression level in roots,followed by shoots,leaves,and culms.The expression level of the QtSKOR2 gene is highest in the roots of Q.tumidinoda and increases over time under 50 mmol/L KCl stress treatment.Additionally,the expression of the QtSKOR2 gene leaves is highest in Q.tumidinoda and increases over time under 250 mmol/L NaCl stress treatment.The results of this study reveal that the QtSKOR2 gene might play a significant role in Q.tumidinoda potassium ion transport under salt stress.

关 键 词：筇竹 钾离子通道 QtSKOR2基因 基因克隆 基因表达 盐胁迫 

分 类 号：R73[医药卫生—肿瘤]