艳山姜纯露对啤酒酵母致热家兔的解热作用及下丘脑NF-κB表达的影响  

Effects of dewdrops from Aplinia zerubet fruits and on antipyretic and hypothalamus NF-κB expression in brewer's yeast-induced fever rabbits

作　　者：伍春凤 吴焕 王冰冰 黄诗萍 韦凤超 吴冰燕 梁子宁[1,2] 许立拔 WU Chun-feng;WU Huan;WANG Bing-bing;HUANG Shi-ping;WEI Feng-chao;WU Bing-yan;LIANG Zi-ning;XU Li-ba

机构地区：[1]广西中医药大学,广西南宁530200 [2]广西壮瑶药重点实验室,广西南宁530200 [3]壮瑶药协同创新中心,广西南宁530200 [4]广西壮族自治区民族药资源与应用工程研究中心,广西南宁530200

出　　处：《饲料研究》2024年第22期73-77,共5页Feed Research

基　　金：广西科技基地和人才专项(项目编号:桂科AD21238031);广西重点研发计划项目(项目编号:桂科AB21196016);广西壮瑶药重点实验室(项目编号:桂科基字[2014] 32号);壮瑶药协同创新中心(项目编号:桂教科研[2013] 20号);广西壮族自治区民族药资源与应用工程研究中心(项目编号:桂发改高技函[2020] 2605号);国家自然科学基金项目(项目编号:82060695);广西一流学科中药学(民族药学)(项目编号:桂教科研[2018] 12号);广西中医药大学科研项目(项目编号:05018028F2)。

摘　　要：试验旨在研究艳山姜纯露(DAF)对啤酒酵母致热家兔的解热作用及下丘脑核因子-κB (NF-κB)表达的影响。除对照组外,60日龄家兔颈背部皮下注射啤酒酵母混悬液(135 mg/kg)诱导发热模型,5 h后,取30只肛温增长值在0.8~3.0℃的家兔,随机分为5组。对照组和模型组灌胃纯净水,布洛芬组(10 mg/kg)灌胃布洛芬混悬液,DAF1组、DAF2组、DAF3组分别灌胃1.8、3.6、7.2 g/kg DAF。结果显示,与模型组相比,DAF2组、DAF3组极显著降低发热家兔肛温(P<0.01),降低血清肿瘤坏死因子-α (TNF-α)、白细胞介素-1β (IL-1β)和白细胞介素-6 (IL-6)水平(P<0.05,P<0.01),降低脑脊髓液环磷酸腺苷(cAMP)和前列腺素E_(2)(PGE_(2))水平(P<0.05或P<0.01),下调下丘脑NF-κB p65蛋白表达。研究表明,添加3.6 g/kg和7.2 g/kg DAF可减轻家兔热应激损伤。The experiment aimed to investigate the antipyretic effects of dewdrops from Apliniazerubetfruits(DAF)and effects on hypothalamus NF-κBexpression in fever rabbits induced by brewer's yeast.60-day-old rabbits except rabbits in control group were subcutaneously injected with brewer's yeast(135 mg/kg)into their back of neck to establish the fever models,and five hours after modeling,30 rabbits with anal temperature gain 0.8~3.0℃were randomly divided into five groups.Rabbits in control group and model group were intragastrically fed with pure water,rabbits in ibuprofen group were intragastrically fed with dose of ibuprofen suspension 10 mg/kg,and rabbits in group DAF1,DAF2,DAF3 were intragastrically fed with dose of 1.8,3.6,7.2 g/kg DAF.The results showed that compared with the model group,the DAF2 and DAF3 groups extremely reduced the rectal temperature of feverish rabbits(P<0.01),decreased the levels of serum tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6)(P<0.05,P<0.01),reduced the levels of cyclic adenosine monophosphate(cAMP)and prostaglandin E_(2)(PGE_(2))in the cerebrospinal fluid(P<0.05 or P<0.01),and downregulated the expression of NF-κB p65 protein in the hypothalamus.The experiment indicated that adding dewdrops from 3.6 g/kg and 7.2 g/kg DAF can alleviate the damage form heat stress.

关 键 词：艳山姜纯露 兔 解热作用 啤酒酵母 内生致热原 NF-κB p65 

分 类 号：S829.1[农业科学—畜牧学]