基于转录组分析的甘薯贮藏根淀粉含量相关SNPs开发  

Mining of SNPs Related to Starch Content in Sweet Potato Storage Roots Based on Transcriptome Analysis

作　　者：秦桢[1] 王庆美[1] 李爱贤[1] 周媛媛 解备涛[1] 段文学[1] 张海燕[1] 李广华 董顺旭[1] 侯夫云[1] Qin Zhen;Wang Qingmei;Li Aixian;Zhou Yuanyuan;Xie Beitao;Duan Wenxue;Zhang Haiyan;Li Guanghua;Dong Shunxu;Hou Fuyun(Crop Research Institute,Shandong Academy of Agricultural Sciences/Scientific Observation and Experimental Station of Tubers and Root Crops in Huang-Huai-Hai Region,Ministry of Agriculture and Rural Affairs,Jinan 250100,China;Sishui Lifeng Food Co.,Ltd.,Sishui 273200,China)

机构地区：[1]山东省农业科学院作物研究所/农业农村部黄淮海薯类科学观测站,山东济南250100 [2]泗水利丰食品有限公司,山东泗水273200

出　　处：《山东农业科学》2024年第12期10-15,共6页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2024MC207);山东省重点研发计划项目(2021LZGC025);泰山产业领军人才工程项目(LJNY202113);国家甘薯产业技术体系项目(CARS-10-GW08);山东省薯类产业技术体系项目(SDAIT-16-04)。

摘　　要：甘薯是重要的粮食和经济作物,贮藏根淀粉含量是甘薯的重要农艺性状,开发与淀粉含量相关的分子标记对于加快育种进程具有重要意义。但甘薯具有自交不亲和特性,基因组是高度杂合的六倍体,增加了开发分子标记的难度。本试验以高淀粉品种漯徐薯8号和低淀粉品种郑薯20及其杂交F1代中6个高淀粉株系和6个低淀粉株系为材料,基于转录组测序分析并结合表型分析,发现44个高淀粉特异的SNPs位点,从中选取9个进行PCR单克隆分析,筛选到3个与高淀粉含量显著相关的位点,分别为chr9.27120209、chr9.27120256和chr9.13675504,用上述12个株系和F_(1)群体中的另外16个株系进行验证,最终得到3个与贮藏根淀粉含量相关的单核苷酸多态性(SNPs)位点。本试验丰富了甘薯分子标记开发的途径,为甘薯贮藏根淀粉含量相关育种提供了可选择的标记。Sweet potato is an important food and economic crop,and the starch content in its storage roots is a crucial agronomic trait.Mining molecular markers related to starch content is of great significance for accelerating the breeding process.However,sweet potato exhibits self⁃incompatibility and has a highly hetero⁃zygous hexaploid genome,which increases the difficulty in developing molecular markers.In this study,high⁃starch variety Luoxushu 8 and low⁃starch variety Zhengshu 20 along with six high⁃starch and six low⁃starch lines from their hybrid F1 generation were used as materials.Based on transcriptome sequencing analysis com⁃bined with phenotypic analysis,44 high⁃starch⁃specific SNPs were identified,and nine of them were selected for PCR monoclonal analysis.Three loci,chr9.27120209,chr9.27120256 and chr9.13675504,were found to be significantly associated with high starch content,which were further validated in the previously 12 selected materials and other 16 lines from the hybrid population.This study enriched the approaches for developing mo⁃lecular markers in sweet potato and provided selectable markers for breeding sweet potato varieties with desired storage root starch content.

关 键 词：甘薯 贮藏根 淀粉含量 单核苷酸多态性标记 转录组分析 

分 类 号：S531.01[农业科学—作物学]