肝细胞肝癌组织MCM4蛋白表达及其与临床病理特征的关系  

MCM4 Protein Expression in Hepatocellular Carcinoma Tissue and Its Relationship with Clinical Pathological Characteristics

作　　者：王超[1] 王彦玲 李丽[1] WANG Chao;WANG Yanling;LI Li(Department of Oncology,Zhengzhou Yihe Hospital Affiliated to Henan University,Zhengzhou 450000,China)

机构地区：[1]河南大学附属郑州颐和医院肿瘤科,河南郑州450000

出　　处：《河南医学研究》2025年第1期18-23,共6页Henan Medical Research

摘　　要：目的探究肝细胞肝癌组织微小染色体维持蛋白4(MCM4)蛋白表达及其与临床病理特征的关系。方法选取2021年1月至2022年2月河南大学附属郑州颐和医院收治的102例行手术切除的肝细胞肝癌患者作为研究对象,采用免疫组织化学法检测癌组织与切缘正常组织MCM4蛋白表达,比较癌组织和切缘正常组织MCM4蛋白阳性表达率;比较不同临床病理特征患者癌组织MCM4蛋白阳性表达率;随访1 a分析癌组织MCM4蛋白表达与生存的关系;采用Kaplan-Meier法进行生存分析,并通过log-rank进行显著性检验。结果肝细胞肝癌组织MCM4蛋白阳性表达率高于切缘正常组织(P<0.05);乙型肝炎表面抗原(HBsAg)阳性、肿瘤淋巴结转移(TNM)分期Ⅲa期、低/未分化、肿瘤最大径≥5 cm、甲胎蛋白(AFP)≥400μg·L^(-1)、有微血管侵犯、有肝硬化患者癌组织MCM4蛋白阳性表达率分别高于HBsAg阴性、TNM分期I~Ⅱ期、高/中分化、肿瘤最大径<5 cm、AFP<400μg·L^(-1)、无微血管侵犯、无肝硬化患者,差异均有统计学意义(P<0.05);随访1 a,患者生存率为78.79%,HBsAg阳性、TNM分期Ⅲa期、低/未分化、AFP≥400μg·L^(-1)、有肝硬化、MCM4蛋白阳性表达均为肝癌患者死亡的危险因素(HR=4.026、5.767、6.430、6.874、4.496、7.588,P<0.05);癌组织MCM4蛋白阳性表达患者1 a生存率低于阴性表达患者(P<0.05)。结论肝细胞肝癌组织MCM4蛋白阳性表达率高于切缘正常组织,癌组织MCM4蛋白表达与HBsAg、TNM分期、分化程度、肿瘤大小、AFP水平、微血管侵犯、肝硬化有关,且癌组织MCM4蛋白阳性表达可降低患者生存率。Objective To explore the relationship between microchromosome maintenance protein 4(MCM4)expression and clinicopathological features in hepatocellular carcinoma.Methods A total of 102 hepatocellular carcinoma patients who underwent surgical resection in Zhengzhou Yihe Hospital Affiliated to Henan University from January 2021 to February 2022 were selected as the research subjects.Immunohistochemical methods were used to detect the expression of MCM4 protein in cancer tissue and normal tissue at the cutting edge,and the positive expression rate of MCM4 protein in cancer tissue and normal tissue at the cutting edge was compared.The positive expression rate of MCM4 protein in cancer tissues of patients with different clinical and pathological characteristics were compared.The relationship between MCM4 protein expression and survival in cancer tissues was analyzed after one year follow-up.Survival analysis was conducted using the Kaplan-Meier method and conduct significance testing through log-rank.Results The positive expression rate of MCM4 protein in hepatocellular carcinoma tissue was higher than that in normal tissue at the cutting edge(P<0.05).The positive expression rate of MCM4 protein in cancer tissue of patients with hepatitis B surface antigen(HBsAg)positive,tumor node metastasis(TNM)stageⅢa,low/undifferentiated,tumor maximum diameter≥5 cm,alpha fetoprotein(AFP)≥400μg·L^(-1),microvascular invasion and liver cirrhosis was higher than HBsAg negative,TNM stageⅠ-Ⅱ,high/medium differentiation,maximum tumor diameter<5 cm,AFP<400μg·L^(-1),no microvascular invasion and no cirrhosis patients,respectively,and there were statistically significant differences(P<0.05).Following up for one year,the survival rate of patients was 78.79%,with HBsAg positive,TNM stageⅢa,low/undifferentiated,AFP≥400μg·L^(-1),cirrhosis,and positive expression of MCM4 protein were all risk factors for death in liver cancer patients(HR=4.026,5.767,6.430,6.874,4.496,7.588,P<0.05).The one year survival rate of patients with po

关 键 词：肝细胞肝癌 微小染色体维持蛋白4 临床病理特征 

分 类 号：R735.7[医药卫生—肿瘤]