右美托咪定对宫颈癌细胞SiHa生物学行为及TLR4/MyD88通路的影响  

Effects of dexmedetomidine on biological behavior and TLR4/MyD88 pathway of cervical cancer SiHa cells

作　　者：娄伦美 马兴龙 吴柳 王曦 张玉清[3] LOU Lun-Mei;MA Xing-Long;WU Liu(Department of Clinical Pharmacy,Zunyi First People's Hospital&The Third Affiliated Hospital of Zunyi Medical University,Zunyi 563000,Guizhou,China)

机构地区：[1]遵义市第一人民医院(遵义医科大学第三附属医院)临床药学部,贵州遵义563000 [2]遵义市第一人民医院(遵义医科大学第三附属医院)重症医学科,贵州遵义563000 [3]石家庄医学高等专科学校病理教研室

出　　处：《中国老年学杂志》2024年第16期3924-3928,共5页Chinese Journal of Gerontology

基　　金：国家自然科学基金青年科学基金项目(82301971)。

摘　　要：目的探讨右美托咪定对宫颈癌细胞SiHa生物学行为及Toll样受体(TLR)4/髓样分化因子(MyD)88通路的影响。方法体外培养人宫颈癌细胞SiHa,取培养至对数生长期的SiHa细胞分为空白组(在DMEM培养基中常规培养Si-Ha细胞)、顺铂组(用含有2.5 mg/L顺铂的DMEM培养基培养SiHa)、右美托咪定低、高(分别用含有3、6μmol/L右美托咪定的DMEM培养基培养SiHa)浓度组。四甲基偶氮唑蓝(MTT)法、平板克隆形成实验、划痕试验、流式细胞术分别检测SiHa细胞增殖率、克隆形成数目、迁移率、凋亡率;实时荧光定量聚合酶链反应(RT-qPCR)和Western印迹分别检测SiHa中TLR4、MyD88 mRNA和蛋白表达水平。结果与空白组相比,顺铂组、右美托咪定低、高浓度组SiHa细胞增殖率、克隆形成数目、迁移率、TLR4、MyD88 mRNA和蛋白表达水平显著降低,凋亡率显著升高(P<0.05);与顺铂组相比,右美托咪定低、高浓度组Si-Ha细胞增殖率、克隆形成数目、迁移率、TLR4、MyD88 mRNA和蛋白表达水平显著升高,凋亡率显著降低(P<0.05);与右美托咪定低浓度组相比,右美托咪定高浓度组SiHa细胞增殖率、克隆形成数目、迁移率、TLR4、MyD88 mRNA和蛋白表达水平显著降低,凋亡率显著升高(P<0.05)。结论右美托咪定能有效抑制SiHa细胞增殖和迁移,促进其凋亡,其机制可能与抑制TLR4/MyD88通路的激活有关。Objective To investigate the effects of dexmedetomidine on biological behavior in cervical cancer SiHa cells and Toll like receptor(TLR)4/myeloid differentiation factor(MyD)88 pathway.Methods Human cervical cancer SiHa cells cultured in vitro,SiHa cells cultured to logarithmic growth phase were divided into blank group(conventional culture of SiHa cells in DMEM medi-um),cisplatin group(SiHa cells cultured in DMEM medium containing 2.5 mg/L cisplatin),low,high(SiHa cells cultured in DMEM medium containing 3,6μmol/L dexmedetomidine)concentration of dexmedetomidine groups.The proliferation rate,number of clones formed,migration rate and apoptosis rate of SiHa cells were detected by methyl thiazolyl tetrazolium(MTT)assay,plate clo-ning assay,scratch assay and flow cytometry,respectively.The mRNA and protein expression levels of TLR4 and MyD88 in SiHa cells were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)and Western blot.Results Compared with blank group,the proliferation rate,number of clones formed,migration rate,TLR4,MyD88 mRNA and protein expression levels of Si-Ha cells in cisplatin group,dexmedetomidine low and high concentration groups were significantly decreased,and apoptosis rate was significantly increased(P<0.05);compared with cisplatin group,the proliferation rate,number of clones formed,migration rate,TLR4,MyD88 mRNA and protein expression levels of SiHa cells in low and high concentration of dexmedetomidine groups were signifi-cantly increased,and apoptosis rate was significantly decreased(P<0.05);compared with low concentration of dexmedetomidine group,the proliferation rate,number of clones formed,migration rate,TLR4,MyD88 mRNA and protein expression levels of SiHa cells in high concentration of dexmedetomidine group were significantly decreased,and apoptosis rate was significantly increased(P<0.05).Conclusions Dexmedetomidine could effectively inhibit the proliferation and migration of SiHa cells and promote their apopto-sis,and its mechanism might be related t

关 键 词：右美托咪定 宫颈癌细胞SiHa Toll样受体4/髓样分化因子88通路 生物学行为 

分 类 号：R737.33[医药卫生—肿瘤]