miRNA-6507增强宫颈癌细胞顺铂敏感性及其机制探讨  

MiRNA-6507 enhances cisplatin sensitivity in cervical cancer cells and its mechanism exploration

作　　者：张海光 赵晶晶 张敏[2] 崔非非 王朝辉[2] 杨君[1] 王国戗[2] ZHANG Haiguang;ZHAO Jingjing;ZHANG Min;CUI Feifei;WANG Zhaohui;YANG Jun;WANG Guoqiang(Department of Gynecology and Obstetrics,the First Affiliated Hospital of Xinxiang Medical University,Henan Xinxiang 453100,China;Clinical Lab,the First Affiliated Hospital of Xinxiang Medical University,Henan Xinxiang 453100,China)

机构地区：[1]新乡医学院第一附属医院妇产科,河南新乡453100 [2]新乡医学院第一附属医院检验科,河南新乡453100

出　　处：《现代肿瘤医学》2025年第1期26-31,共6页Journal of Modern Oncology

基　　金：新乡医学院第一附属医院青年科学基金(编号:QN-2021-A05,QN-2021-B14)。

摘　　要：目的:探讨miRNA-6507对宫颈癌细胞顺铂敏感性的影响及其机制。方法:通过TCGA数据库下载宫颈癌相关miRNA数据及临床数据,分析癌旁组织与癌组织间miRNA表达差异,并分析得到与患者生存期相关的miRNA;通过RT-qPCR验证所得miRNA在宫颈正常细胞与宫颈癌细胞间表达差异。选取与患者5年生存率相关的miRNA,将miRNA模拟物及对照转染至宫颈癌细胞系中,MTT检测细胞增殖,对转染后的细胞加入顺铂,检测细胞对顺铂的敏感性。结果:分析发现TCGA数据库宫颈癌标本中共有256个肿瘤组织,2个癌旁组织;通过edgeR分析发现宫颈癌相关的1881个miRNA中肿瘤组织同癌旁组织相比,表达上调的有19个,表达下降的有70个;对这89个miRNA分别分为高表达组和低表达组,进行生存分析结果发现,miRNA-140、miRNA-145、miRNA-200c、miRNA-6507与患者的生存期相关,miRNA-140、miRNA-145、miRNA-6507高表达患者生存期较长,miRNA-200c高表达患者生存期较短。RT-qPCR结果显示,在宫颈癌细胞系Caski、HCC94、Siha中miRNA-140的表达同宫颈细胞H8相比显著升高,在C33A和Hela细胞中miRNA-140无显著性差异。在所有的宫颈癌细胞中miRNA-145的表达均下降,该结果与生存分析一致;在宫颈癌细胞Caski和HCC94中miRNA-200c的表达上调,这与生存分析的结果相同,在C33A、Hela和Siha细胞中miRNA-200c的表达没有显著变化;在验证的5种宫颈癌细胞中miRNA-6507的表达量均低于正常细胞系H8,该结果同生存分析一致。MTT结果显示,C33A和Siha转染miRNA-6507模拟物后,其细胞活力均未受到影响,对转染后的细胞进行顺铂处理,结果显示转染miRNA-6507的细胞活力降低。结论:miRNA-6507可增强宫颈癌细胞的顺铂敏感性,有望成为宫颈癌治疗的候选miRNA靶点。Objective:Exploring the effect and mechanism of miRNA-6507 on cisplatin sensitivity in cervical cancer cells.Methods:The miRNA and clinical data related to cervical cancer were downloaded from the TCGA database to analyze the miRNA expression differences between adjacent and cancerous tissues,the miRNA related to patient survival was further analysis.The expression difference of miRNA between normal cervical cells and cervical carcinoma cells was verified by RT-qPCR.The miRNA related to the 5-year survival rate of patients was chosen,and the miRNA mimics and controls were transfected into cervical cancer cell lines.MTT was used to detect cell viability,and cell sensitivity to cisplatin was detected after cisplatin was added to transfected cells.Results:There were 256 tumor tissues and 2 para-cancer tissues in TCGA database.edgeR analysis showed that among 1881 miRNA associated with cervical cancer,19 were up-regulated and 70 were down-regulated in tumor tissues compared with adjacent tissues.Each of the 89 miRNA were divided into high expression group and low expression group for survival analysis,results showed that miRNA-140,miRNA-145,miRNA-200c,and miRNA-6507 were correlated with the survival of patients.The high expression of miRNA-140,miRNA-145 and miRNA-6507 can improve the survival of patients,and the high expression of miRNA-200c can reduce the survival of patients.RT-qPCR results showed that the expression of miRNA-140 in cervical cancer cell lines Caski,HCC94 and Siha was significantly higher than that in cervical cancer cells H8,but there was no significant difference in C33A and Hela cells.The expression of miRNA-145 was decreased in all cervical cancer cells,which was consistent with survival analysis.The expression of miRNA-200c was up-regulated in Caski and HCC94 cervical cancer cells,which was the same as the results of survival analysis,but there was no significant change in the expression of miRNA-200c in C33A,Hela and Siha cells.The expression level of miRNA-6507 in the five cervical cancer cel

关 键 词：宫颈癌 5年生存率 顺铂敏感性 MIRNA 

分 类 号：R737.33[医药卫生—肿瘤]