阿魏酸对高糖诱导受损的人间充质干细胞生物学功能、凋亡和HGF分泌的影响  

Effects of Ferulic Acid on Biological Function,Apoptosis and HGF Secretion of Human Mesenchymal Stem Cells Damaged by High Glucose

作　　者：刘畅 潘晴 彭光霖 刘向男 胡清睿 武闻静 郭子莘 胡敏霞 宾马赢 熊武[2] LIU Chang;PAN Qing;PENG Guanglin;LIU Xiangnan;HU Qingrui;WU Wenjing;GUO Zixin;HU Minxia;BIN Maying;XIONG Wu(Department of Encephalopathy(Neuromedicne),Ningxiang Hospital of Traditional Chinese Medicine,Changsha 410600,China;Department of Burn and Plastic Surgery,the First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;School of Integrated Traditional Chinese and Western Medicine,Moxibustion and Tuina,Hunan University of Chinese Medicine,Changsha 410208,China;School of Acupuncture,Moxibustion and Tuina,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]宁乡市中医医院脑病(神经医学)科,长沙410600 [2]湖南中医药大学第一附属医院烧伤整形科,长沙410007 [3]湖南中医药大学中西医结合学院,长沙410208 [4]湖南中医药大学针灸推拿学院,长沙410208

出　　处：《医学综述》2025年第3期377-384,共8页Medical Recapitulate

基　　金：国家自然科学基金(81904217);长沙市自然科学基金(kq2403212)。

摘　　要：目的探讨阿魏酸对高糖诱导受损的人间充质干细胞(MSCs)生物学功能、凋亡和肝细胞生长因子(HGF)分泌的影响。方法体外分离培养人脐带血MSCs,取第4代细胞进行成骨、成软骨和成脂诱导分化鉴定。将鉴定成功的MSCs用30 mmol/L的葡萄糖预培养120 h,用不同浓度阿魏酸(0、1、2、4、8、16 mg/L)进行干预,确定阿魏酸促进高糖诱导受损MSCs增殖的最佳浓度。将高糖诱导受损MSCs按照随机数字表法分为受损MSCs组与阿魏酸干预组,同时设置正常MSCs组,阿魏酸干预组用最佳浓度的阿魏酸干预,受损MSCs组和正常MSCs组用等容量磷酸盐缓冲液处理。三组培养24 h后,比较各组MSCs增殖、黏附、迁移和凋亡情况;通过酶联免疫吸附测定法检测各组HGF的含量。结果阿魏酸促高糖诱导受损MSCs增殖的最佳浓度为2 mg/L。与正常MSCs组相比,受损MSCs组细胞增殖OD值、细胞黏附数以及迁移实际宽度均明显降低(均P<0.01);与受损MSCs组相比,阿魏酸干预组细胞增殖OD值、细胞黏附数和迁移实际宽度均明显增加[0.35±0.02比0.25±0.03、(27.00±4.32)个比(10.17±0.53)个、(15.37±0.90)μm比(7.30±2.03)μm](均P<0.01)。与正常MSCs组相比,受损MSCs组细胞凋亡率升高(P<0.01);与受损MSCs组相比,阿魏酸干预组细胞凋亡率明显降低[(10.79±0.78)%比(15.33±0.97)%](P<0.01)。与正常MSCs组相比,受损MSCs组HGF含量明显降低(P<0.01);与受损MSCs组相比,阿魏酸干预组HGF含量明显升高[(242±31)ng/L比(52±6)ng/L](P<0.01)。结论阿魏酸能够保护高糖诱导受损MSCs,增强其生物学功能和分泌功能,减少其凋亡。Objective To investigate the effects of ferulic acid on biological function,apoptosis and secretion of hepatocyte growth factor(HGF)in high glucose induced damaged human umbilical cord blood mesenchymal stem cells(MSCs).Methods Human umbilical cord blood MSCs were isolated and cultured in vitro.The P4 passage cells were identified for osteogenic,chondrogenic and adipogenic differentiation.The identified MSCs were pre-cultured with 30 mmol/L glucose for 120 h,and then intervened with different concentrations of ferulic acid(0,1,2,4,8,16 mg/L)to determine the optimal concentration of ferulic acid to promote the proliferation of high glucose damaged MSCs.The MSCs were randomly divided into a damaged MSCs group and a ferulic acid intervention group,while a normal MSCs group was set up.The ferulic acid intervention group was intervened with the optimal concentration of ferulic acid,and the damaged MSCs group and normal MSCs group were treated with equal volume phosphate buffer.After 24 hours of culture,the proliferation,adhesion,migration and apoptosis of MSCs in each group were compared,and the content of HGF in each group was detected by enzyme linked immunosorbent assay.Results The optimal concentration of ferulic acid to promote the proliferation of high glucose damaged MSCs was 2 mg/L.Compared with normal MSCs group,cell proliferation OD value,cell adhesion number and actual width of migration in the damaged MSCs group were significantly decreased(all P<0.01).Compared to the damaged MSCs group,cell proliferation OD value,cell adhesion number and actual migration width were significantly increased in the ferulic acid intervention group[0.35±0.02 vs 0.25±0.03,27.00±4.32 vs 10.17±0.53,(15.37±0.90)μm vs(7.30±2.03)μm](all P<0.01).Compared with normal MSCs group,the apoptosis rate of the damaged MSCs group was increased(P<0.01).Compared with the damaged MSCs group,the apoptosis rate of the ferulic acid intervention group was significantly decreased[(10.79±0.78)%vs(15.33±0.97)%](P<0.01).Compared with normal MS

关 键 词：阿魏酸 间充质干细胞 高糖环境 生物学功能 肝细胞生长因子 

分 类 号：R264[医药卫生—中医外科学]