血清Bmi-1联合HPV-DNA分型检测对宫颈癌的诊断价值  

Diagnostic value of serum Bmi-1 combined with HPV-DNA typing in cervical cancer

作　　者：王占英 刘晓梅 王智慧 WANG Zhan-ying;LIU Xiao-mei;WANG Zhi-hui(Department of Neurology,Daqing Longnan Hospital(The Fifth Affiliated Hospital of Qiqihar Medical College),Daqing Heilongjiang 163453,China)

机构地区：[1]大庆龙南医院(齐齐哈尔医学院附属第五医院)检验科,黑龙江大庆163453

出　　处：《临床和实验医学杂志》2024年第24期2644-2647,共4页Journal of Clinical and Experimental Medicine

基　　金：黑龙江省卫健委科研课题(编号:SJGZY2022004)。

摘　　要：目的分析宫颈癌患者应用血清B细胞特异性莫洛尼白血病毒插入位点1(Bmi-1)联合人乳头状瘤病毒(HPV)-DNA分型检测的结果以及临床诊断价值。方法回顾性纳入2022年1月至2024年6月到大庆龙南医院就诊的80例宫颈癌患者为研究对象,设为观察组。以手术病理检测结果为金标准将观察组细分为恶性组(n=36)和良性组(n=44),同时选取同期到本院进行健康体检的40名女性,设为对照组。所有研究对象均接受血清Bmi-1、HPV-DNA分型检测,对比分析各组患者的血清Bmi-1、HPV-DNA分型检测结果,两者阳性表达与临床病理参数的关系及单独检测、联合检测对宫颈癌的诊断价值。结果三组研究对象的血清Bmi-1表达及HPV-DNA分型检测结果比较,差异均有统计学意义(P<0.05),其中,恶性组血清Bmi-1表达及HPV-DNA总阳性率分别为0.42±0.12、91.67%,均显著高于良性组(0.29±0.08、27.27%)、对照组(0.21±0.07、7.50%),差异均有统计学意义(P<0.05)。Spearman相关分析结果显示,血清Bmi-1表达与HPV-DNA分型检测结果呈正相关性(r=0.492,P<0.05)。以手术病理检测为金标准,血清Bmi-1、HPV-DNA分型联合检测诊断宫颈癌的准确度、灵敏度、特异度均高于单一检测,差异均有统计学意义(P<0.05);血清Bmi-1表达与宫颈癌临床分期、淋巴结转移有关(P<0.05),与年龄、病理分型无关(P>0.05);HPV-DNA分型检测结果与年龄、宫颈癌病理分型、临床分期及淋巴结转移均无关(P>0.05)。结论宫颈癌患者的血清Bmi-1表达上调,HPV-DNA分型以16、18等高危型为主,血清Bmi-1与HPV-DNA分型检测结果呈正相关性,在宫颈癌发生、进展过程中可能发挥出协同作用,两者联合对宫颈癌的诊断效能较高。Objective To analyze the results and clinical diagnostic value of serum B cell specific moloney leukemia virus insertion site 1(Bmi-1)combined human papilloma virus(HPV)-DNA typing in patients with cervical cancer.Methods A total of 80 patients with cervical cancer who visited Daqing Longnan Hospital from January 2022 to June 2024 were retrospectively included as the study objects and set as the observation group.The observation group was subdivided into the malignant group(n=36)and the benign group(n=44)based on the results of surgical and pathological tests as the gold standard.At the same time,40 women who went to our hospital for physical examination in the same period were selected as the control group.All patients received serum Bmi-1 and HPV-DNA typing tests,and the serum Bmi-1 and HPV-DNA typing test results of each group,as well as the relationship between positive expression of the two and clinicopathological parameters,and the diagnostic value of single detection and combined detection were compared and analyzed.Results The serum Bmi-1 expression and HPV-DNA typing test results of the three groups were compared,and the differences were statistically significant(P<0.05).Among them,the serum Bmi-1 expression and HPV-DNA total positive rate in the malignant group were 0.42±0.12 and 91.67%,respectively,which were significantly higher than those in the benign group(0.29±0.08,27.27%)and the control group(0.21±0.07,7.50%),and the differences were statistically significant(P<0.05).Spearman correlation analysis showed that serum Bmi-1 expression was positively correlated with HPV-DNA typing results(r=0.492,P<0.05).With surgical pathologic detection as the gold standard,the accuracy,sensitivity and specificity of serum Bmi-1 and HPV-DNA typing were higher than that of single detection in the diagnosis of cervical cancer,and the differences were statistically significant(P<0.05).Serum Bmi-1 expression was correlated with clinical stage and lymph node metastasis of cervical cancer(P<0.05),but not with age or pa

关 键 词：宫颈肿瘤 诊断 BMI-1 HPV-DNA分型 相关性 

分 类 号：R73[医药卫生—肿瘤]