结肠癌患者血清miR-152-5p、miR-503-5p的表达水平及与预后的关系  

Serum miR-152-5p and miR-503-5p expression levels in colon cancer patients and their relationship with prognosis

作　　者：孙萌[1] 张兴[1] 张刘平[1] 李甫根 钱川[1] 许政文 SUN Meng;ZHANG Xing;ZHANG Liuping;LI Fugen;QIAN Chuan;XU Zhengwen(Department of Gastrointestinal Surgery,Suining Central Hospital,Suining 629000,China)

机构地区：[1]遂宁市中心医院胃肠外科,四川遂宁629000

出　　处：《临床肿瘤学杂志》2024年第9期868-872,共5页Chinese Clinical Oncology

摘　　要：目的探讨微小RNA-152-5p(miR-152-5p)和微小RNA-503-5p(miR-503-5p)在结肠癌患者血清中的表达情况,以及二者与结肠癌临床病理特征和预后的关系。方法收集2017年7月至2020年7月120例结肠癌患者的临床资料,同时收集120例健康体检者作为对照。实时荧光定量PCR(qPCR)法检测血清miR-152-5p和miR-503-5p的表达水平。Pearson法分析两者表达水平的相关性,Kaplan-Meier法分析二者表达水平与结肠癌患者预后的关系,Cox比例风险回归模型分析影响结肠癌患者预后的因素。结果结肠癌组血清miR-152-5p的相对表达量为1.73±0.22,高于对照组的0.99±0.19(P<0.05),结肠癌组血清miR-503-5p的相对表达量为0.61±0.10,低于对照组的0.98±0.11(P<0.05)。miR-152-5p与miR-503-5p在结肠癌患者血清中的表达水平呈负相关(r=-0.411,P<0.05)。血清miR-152-5p、miR-503-5p表达与TNM分期、淋巴结转移和分化程度有关(P<0.05);与性别、年龄、肿瘤直径和辅助放化疗无关(P>0.05)。结肠癌患者血清miR-152-5p高表达组的3年生存率为40.6%,低于低表达组的57.1%(P=0.004);miR-503-5p高表达组的3年生存率为59.6%,高于低表达组的39.7%(P=0.001)。Cox回归分析显示,miR-152-5p、miR-503-5p、淋巴结转移、分化程度和TNM分期为影响结肠癌患者预后的独立因素(P<0.05)。结论结肠癌患者的血清miR-152-5p表达上调,miR-503-5p表达下调,二者表达水平呈负相关,且可能与结肠癌患者的预后有关。Objective To investigate the expression levels of serum microRNA-152-5p(miR-152-5p)and microRNA-503-5p(miR-503-5p)of colon cancer patients and their relationship with clinicopathological features and prognosis.Methods The clinical data of 120 colon cancer patients and 120 healthy subjects from July 2017 to July 2020 were collected.The expression levels of serum miR-152-5p and miR-503-5p were detected by real-time quantitative PCR(qPCR).The correlation between the expression levels of the two items was analyzed by Pearson's method,and the relationship between the expression levels and prognosis was analyzed by Kaplan-Meier method.The factors affecting the prognosis of colon cancer patients were analyzed by Cox proportional hazards regression model.Results The relative expression level of miR-152-5p in colon cancer patients was 1.73±0.22,higher than 0.99±0.19 of the control group(P<0.05).The relative expression level of miR-503-5p in colon cancer patients was 0.61±0.10,lower than 0.98±0.11 of the control group(P<0.05).The expression levels of serum miR-152-5p and miR-503-5p of colon cancer patients were negatively correlated(r=-0.411,P<0.05).The expression levels of miR-152-5p,miR-503-5p,TNM staging,lymph node metastasis,and differentiation degree were related to prognosis(P<0.05),while those of gender,age,tumor diameter,and adjuvant radiotherapy and chemotherapy were not(P>0.05).The 3-year survival rate of colon cancer patients with high expression of miR-152-5p was 40.6%,lower than 57.1%of the low expression group(P=0.004);the 3-year survival rate of the group with high expression of miR-503-5p was 59.6%,higher than 39.7%of the low expression group(P=0.001).Cox regression analysis showed that miR-152-5p,miR-503-5p,lymph node metastasis,differentiation degree,and TNM stage were independent prognostic factors affecting the survival of colon cancer patients(P<0.05).Conclusion The expression of miR-152-5p is upregulated and that of miR-503-5p is downregulated in colon cancer patients,and the two expression levels

关 键 词：结肠癌 微小RNA-152-5p 微小RNA-503-5p 预后 

分 类 号：R735.3[医药卫生—肿瘤]