黄腐酚对孕期感染子代大鼠精神分裂症样行为的影响及机制  

Effect of xanthohumol on schizophrenia-like behaviors in the offspring of infected pregnant rats and related mechanisms

作　　者：程淑敏 宋盟 李文强[1,2] 吕路线 CHENG Shumin;SONG Meng;LI Wenqiang;LYU Luxian(The Second Clinical College of Xinxiang Medical University,Xinxiang 453002,Henan Province,China;Henan Provincial Key Laboratory of Biological Psychiatry,Xinxiang 453002,Henan Province,China)

机构地区：[1]新乡医学院第二临床学院,河南新乡453002 [2]河南省生物精神病学重点实验室,河南新乡453002

出　　处：《新乡医学院学报》2025年第1期1-8,共8页Journal of Xinxiang Medical University

基　　金：国家自然科学基金资助项目(编号:82171498);河南省生物精神病学重点实验室开放课题(编号:ZDSYS2022002)。

摘　　要：目的探讨黄腐酚(Xn)对孕期感染子代大鼠精神分裂症样行为及小胶质细胞活化和小胶质细胞M1/M2极化的影响。方法选择20只受孕成功的孕鼠,在孕9.5 d时随机将其分为对照组和聚肌胞苷酸(Poly I:C)组,每组10只;Poly I:C组孕鼠经尾静脉注射10 mg·kg^(-1) Poly I:C,对照组孕鼠经尾静脉注射等体积的无菌生理盐水。应用酶联免疫吸附试验(ELISA)法检测2组孕鼠血浆中肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平。2组孕鼠的子代大鼠于出生21 d时进行离乳,随机取对照组每只孕鼠的子代大鼠2只,共20只,并随机将其分为生理盐水组(n=10)和Xn生理盐水组(n=10);随机取Poly I : C组每只孕鼠的子代大鼠2只,共20只,并随机将其分为模型组(n=10)和Xn模型组(n=10)。于出生后56~65 d,每天分别从不同组笼中选取1只子代大鼠进行行为学检测,Xn模型组和Xn生理盐水组子代大鼠均于行为学检测开始前1.5 h腹腔注射10 mg·kg^(-1) Xn,模型组和生理盐水组子代大鼠于行为学检测开始前1.5 h腹腔注射等体积的无菌生理盐水。采用前脉冲抑制实验(PPI)检测4组子代大鼠的惊吓反应抑制能力,旷场实验检测4组子代大鼠自发活动和焦虑样情绪,高架十字迷宫实验检测4组子代大鼠的焦虑样行为,Y迷宫实验检测4组子代大鼠的记忆功能,被动规避实验检测4组子代大鼠的抑制性回避反应能力;应用蛋白质免疫印迹法检测4组子代大鼠海马组织中分化群(CD)206、几丁质酶3样蛋白3(YM)、环氧化酶-2(COX-2)蛋白相对表达量,ELISA法检测4组子代大鼠TNF-α水平。结果Poly I:C组孕鼠血浆TNF-α、IL-6水平显著高于对照组(P<0.05)。70、80、85 dB时,模型组子代大鼠PPI抑制率显著低于生理盐水组,Xn生理盐水组及Xn模型组子代大鼠PPI抑制率显著高于模型组(P<0.05);生理盐水组、Xn生理盐水组及Xn模型组子代大鼠PPI抑制率比较差异无统计学意义(P>0.05)。Xn生理�Objective To explore the effects of xanthohumol(Xn)on schizophrenia-like behaviors,microglial activation,and microglial M1/M2 polarization in the offspring of pregnant rats infected during pregnancy.Methods A total of 20 successfully conceived mice were selected and then randomly divided into a control group and a polyinosinic-polycytidylic acid(Poly I:C)group at 9.5 days of gestation,with 10 mice in each group.The pregnant mice in the Poly I:C group were injected 10 mg·kg^(-1) Poly I:C through the tail vein,while those in the control group were injected an equal volume of sterile saline through the tail vein.The levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in the plasma of pregnant mice in the two groups were detected by enzyme linked immunosorbent assay(ELISA).Offspring rats of the two groups were weaned on the 21st after birth.Two random offspring rats were selected from each pregnant rat of the control group,totaling 20 offspring rats,which were then randomly divided into a saline group(n=10)and a Xn saline group(n=10).Similarly,20 offspring rats were randomly selected from pregnant rats of the Poly I : C group,with 2 offspring rats taken from each pregnant rat.They were then randomly divided into a model group(n=10)and a Xn model group(n=10).From the 56th to 65th day after birth,one offspring rat of different groups was taken for behavioral testing every day.Offspring rats in both Xn model and Xn saline groups were intraperitoneally injected 10 mg·kg^(-1) Xn,while those in both the model and saline groups were intraperitoneally injected an equal volume of sterile physiological saline at 1.5 hours before behavioral testing.The prepulse inhibition experiment(PPI)test was used to assess the inhibitory ability of the four groups of offspring rats to startle reaction.The open field experiment was used to evaluate the spontaneous activity and anxiety-like emotions of offspring rats respectively.The elevated plus maze,Y-maze,and passive avoidance tests were used to detect the anxiety-like beha

关 键 词：黄腐酚 小胶质细胞 炎症 精神分裂症 

分 类 号：R749.3[医药卫生—神经病学与精神病学]