青藏高原环境下大鼠骨骼微结构的基础研究  

Basic Research on the Microstructure of Rat Bones in the High-Altitude Environment of Qinghai-Tibet Plateau

作　　者：赵思斯 王哲涛 尹红科 王春华 索靖航 梁博深 王磊[5] 郜发宝[1,5] ZHAO Sisi;WANG Zhetao;YIN Hongke;WANG Chunhua;SUO Jinghang;LIANG Boshen;WANG Lei;GAO Fabao(Department of Radiology,West China Hospital,Sichuan University,Chengdu 610041,China;Department of Radiology,Sichuan Clinical Research Center for Cancer,Sichuan Cancer Hospital&Institute,Sichuan Cancer Center,and Affiliated Cancer Hospital of University of Electronic Science and Technology of China,Chengdu 610041,China;Jiaxing University,Jiaxing 314001,China;Yunnan University of Chinese Medicine,Kunming 650500,China;Department of Molecular Imaging Laboratory,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西医院放射科,成都610041 [2]四川省肿瘤临床医学研究中心、四川省肿瘤医院研究所、四川省癌症防治中心、电子科技大学附属肿瘤医院影像科,成都610041 [3]嘉兴大学,嘉兴314001 [4]云南中医药大学,昆明650500 [5]四川大学华西医院分子影像研究室,成都610041

出　　处：《四川大学学报(医学版)》2024年第6期1469-1476,共8页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.81930046);西藏自治区科学技术厅重点研发计划(No.XZ202402ZY0003)资助。

摘　　要：目的在真实的高原环境建立低压缺氧大鼠模型,应用Micro CT、血液生化、病理等多种手段,了解真实高原环境对于大鼠骨量以及骨微结构的影响,并探讨其可能的机制。方法将SD大鼠运送至青海玉树高原实验室(海拔4250 m),分别饲养4、8、18个月后,将动物运回成都四川大学华西医院分子影像实验室(海拔500 m),与同期对照组大鼠进行相关检测。检测方法包括:血液生化学;影像学Micro CT;病理学ELISA、Western blot、HE和TRAP染色等病理检查。结果与同期对照组大鼠相比,高原饲养4个月(高原-4)、高原饲养18个月(高原-18)组大鼠体质量下降〔高原-4组vs.平原-4组:(513.75±35.10)g vs.(649.18±60.03)g,P<0.01;高原-18组vs.平原-18组:(535.58±66.65)g vs.(670.86±44.96)g,P<0.01〕;高原饲养8个月(高原-8)组、高原-18组血清中Ca^(2+)浓度较对照组升高〔高原-8组vs.平原-8组:(2.48±0.09)mmol/L vs.(2.38±0.07)mmol/L,P<0.05;高原-18组vs.平原-18组:(2.55±0.11)mmol/L vs.(2.13±0.27)mmol/L,P<0.05〕,P^(3+)差异无统计学意义。骨代谢指标Ⅰ型胶原交联羧基末端肽(cross-linked carboxy-terminal telopeptide of type Ⅰ collagen,CTX-Ⅰ):高原各组较平原组均增高〔高原-4组vs.平原-4组:(1.44±0.08)ng/mL vs.(0.70±0.13)ng/mL,P<0.01;高原-8组vs.平原-8组:(1.52±0.10)ng/mL vs.(0.75±0.10)ng/mL,P<0.01;高原-18组vs.平原-18组:(2.70±0.13)ng/mL vs.(1.94±0.15)ng/mL,P<0.01〕。此外CT结果显示高原3组骨小梁的骨体积分数降低〔高原-4组vs.平原-4组:(7.48±2.35)%vs.(10.40±2.93)%,P<0.05;高原-8组vs.平原-8组:(7.17±2.68)%vs.(10.09±2.95)%,P<0.05;高原-18组vs.平原-18组:(2.90±2.91)%vs.(8.68±4.11)%,P<0.01〕以及骨小梁分离度增大〔高原-4组vs.平原-4组:(0.70±0.12)mm vs.(0.60±0.06)mm,P<0.05;高原-8组vs.平原-8组:(0.68±0.07)mm vs.(0.59±0.05)mm,P<0.01;高原-18组vs.平原-18组:(0.80±0.09)mm vs.(0.70±0.09)mm,P<0.05〕。TRAP染色高原-4、高原-18组破骨细胞增多,Western bloObjective To establish a hypobaric hypoxia rat model in a real high-altitude environment,to investigate the effects of the real high-altitude environment on rat bone mass and bone microstructure using multiple methods such as Micro CT,blood biochemistry,and pathology,and to explore the potential mechanisms involved.Methods Sprague Dawley(SD)rats were transported to the Yushu Plateau Laboratory(at 4250 m above sea level)in Qinghai Province and kept there for 4,or 8,or 18 months.These groups were designated as H-4,H-8,and H-18,respectively.Upon completion of the high-altitude exposure,these animals were transported to the Molecular Imaging Laboratory,West China Hospital,Sichuan University(at 500 m above sea level)in Chengdu for relevant testing and comparison with the control animals raised in a low-altitude environment for the same durations(designated L-4,L-8,and L-18).The tests performed included blood biochemistry,Micro CT imaging,and pathological assessments such as ELISA,Western blot,and HE and TRAP staining.Results Compared with that of the control group,the body mass of rats in the H-4 and H-18 groups decreased significantly(H-4 group vs.L-4 group:[513.75±35.10]g vs.[649.18±60.03]g,P<0.01;H-18 group vs.L-18 group:[535.58±66.65]g vs.[670.86±44.96]g,P<0.01).The serum Ca^(2+)concentration was higher in the H-8 group and H-18 group compared to that in the control group(H-8 group vs.L-8 group:[2.48±0.09]mmol/L vs.[2.38±0.07]mmol/L,P<0.05;H-18 group vs.L-18 group:[2.55±0.11]mmol/L vs.[2.13±0.27]mmol/L,P<0.05).No statistically significant difference was observed in the concentration of P^(3+).Bone metabolism indicator crosslinked carboxy-terminal telopeptide of typeⅠcollagen(CTX-Ⅰ)was significantly increased in all high-altitude groups compared to the low-altitude groups(H-4 group vs.L-4 group:[1.44±0.08]ng/mL vs.[0.70±0.13]ng/mL,P<0.01;H-8 group vs.L-8 group:[1.52±0.10]ng/mL vs.[0.75±0.10]ng/mL,P<0.01;H-18 group vs.L-18 group:[2.70±0.13]ng/mL vs.[1.94±0.15]ng/mL,P<0.01).In addition,CT results sh

关 键 词：低压缺氧 骨微结构 Micro CT 骨代谢 

分 类 号：R73[医药卫生—肿瘤]