胃癌组织Skp2、D-D、p-STAT3表达及临床意义  

Expressions and clinical significances of plasma D-D,Skp2 and p-STAT3 in tumor tissues of gastric cancer

作　　者：陈睿攀 张彤 张美宝 王文月 李晓伟 CHEN Rui-pan;ZHANG Tong;ZHANG Mei-bao;WANG Wen-yue;LI Xiao-wei(The Second People's Hospital of Hengshui,Hengshui,Hebei 053000,China)

机构地区：[1]衡水市第二人民医院康复医学科,河北衡水053000 [2]衡水市第二人民医院肿瘤三科,河北衡水053000

出　　处：《中华医院感染学杂志》2024年第24期3737-3741,共5页Chinese Journal of Nosocomiology

基　　金：河北省医学科学研究重点课题基金资助项目(20150248)。

摘　　要：目的探究胃癌组织S期激酶相关蛋白2(Skp2)、D-二聚体(D-D)、磷酸化信号转导和转录激活因子3(p-STAT3)表达及临床意义。方法以2021年1月-2022年12月衡水市第二人民医院肿瘤科行胃癌手术患者200例为胃癌组,并以消化科200例胃良性病变患者为良性组。以免疫比浊法检测患者血浆D-D水平,采用免疫组化法检测患者胃组织Skp2、p-STAT3表达,分析各指标与胃癌病理特征的关系,并应用受试者工作特性(ROC)曲线分析各指标联合检测对胃癌及癌细胞远处转移的诊断价值。结果胃癌组Skp2、p-STAT3阳性表达率及血浆D-D水平高于良性组(P<0.05)。组间胃癌组织Skp2、p-STAT3阳性表达率及血浆D-D水平比较,幽门螺杆菌(Hp)阳性患者高于Hp阴性(P<0.05),肿瘤最大径≥5 cm患者高于肿瘤最大径≤5 cm(P<0.05),肿瘤低分化患者高于肿瘤高中分化(P<0.05),TNM分期Ⅲ~Ⅳ期患者高于Ⅰ~Ⅱ期(P<0.05),癌细胞浸润全层至浆膜患者高于仅浸润至肌层(P<0.05),已出现淋巴结转移或远处转移患者高于未出现淋巴结转移或远处转移(P<0.05)。联合检测诊断胃癌及胃癌远处转移的曲线下面积(AUC)大于胃组织Skp2、p-STAT3表达及血浆D-D水平单独检测(P<0.05)。结论胃癌组织Skp2、p-STAT3表达、血浆D-D水平变化与Hp感染、肿瘤分化程度、浸润深度等病理特征有关,且各指标联合检测对胃癌及癌细胞远处转移具有诊断价值。OBJECTIVE To investigate the expression and clinical significance of S-phase kinase related protein 2(SKP2),D-dimer(D-D),phosphorylated signal transducer and activator of transcription 3(p-STAT3)in tissues of gastric carcinoma.METHODS The plasma and tissues of gastric cancer and benign diseases were collected from the 200 patients diagnosed as gastric cancer in oncology department and 200 patients with benign gastric diseases in the department of gastroenterology,the Second People's Hospital of Hengshui from Jan.2021 to Dec.2022.The levels of plasma D-D were detected by immunoturbidimetry,the expressions of SKP2 and p-STAT3 by immuno-histochemistry,and the diagnostic values of the indicators were analyzed by receiver operating characteristic(ROC)curve.RESULTS The positive rates of SKP2 and p-STAT3 and plasma D-D level in gastric cancer group were higher than those in benign group(P<0.05),higher in Helicobacter pylori positive patients than those in negative ones(P<0.05),higher in patients with tumor diameter≥5 cm than those with tumor≤5 cm(P<0.05),higher in patients with low differentiation than those with high or middle differentiation(P<0.05),higher in patients with TNM StageⅢtoⅣthan those with TNM Stage Ⅰ to Ⅱ(P<0.05),higher in the patients with cancer cells infiltrating to serosa than those infiltrating only to muscularis(P<0.05),higher in the patients with lymph node metastasis or distant metastasis than those without lymph node metastasis or distant metastasis(P<0.05).The area under the curve(AUC)of combined detection of SKP2 and p-STAT3 and plasma D-D level for the diagnosis of gastric cancer and its distant metastasis was larger than that of the indicator alone(P<0.05).CONCLUSION The expression changes of Skp2 and p-STAT3 in gastric cancer tissues and plasma D-D level are related to pathological features such as H.pylori infection,tumor differentiation,and depth of invasion;therefore,the combination of these indicators has a diagnostic value for gastric cancer and its distant metastasis.

关 键 词：幽门螺杆菌感染 胃癌 癌组织 S期激酶相关蛋白2 D-二聚体 磷酸化信号转导和转录激活因子3 

分 类 号：R735.2[医药卫生—肿瘤]