EBV感染对慢性牙周炎外周血miR-155和Th17/Treg表达的影响  

Effect of EBV infection on miR-155 and Th17/Treg expression in peripheral blood of patients with chronic periodontitis

作　　者：于彬 何继红 黄星月 YU Bin;HE Ji-hong;HUANG Xing-yue(Deyang Stomatological Hospital,Deyang,Sichuan 618000,China)

机构地区：[1]德阳市口腔医院儿童口腔科,四川德阳618000

出　　处：《中华医院感染学杂志》2024年第24期3779-3782,共4页Chinese Journal of Nosocomiology

基　　金：四川省医学科研基金资助项目(S21029)。

摘　　要：目的探讨EB病毒(EBV)感染对慢性牙周炎外周血微小RNA-155(miR-155)、辅助性T细胞17/调节性T细胞(Th17/Treg)表达的影响。方法选取2022年1月-2023年12月德阳市口腔医院收治的66例慢性牙周炎患者为研究对象,根据是否发生EBV感染分为EBV感染组21例、非EBV感染组45例,收集两组临床资料,通过实时荧光定量聚合酶链式反应法和流式细胞术检测两组外周血miR-155水平、Treg细胞、Th17细胞及Th17/Treg比值,受试者工作特征(ROC)曲线分析miR-155、Treg细胞、Th17细胞及Th17/Treg对慢性牙周炎EBV感染的预测价值。结果EBV感染组吸烟史比例(57.14%)、菌斑指数(2.58±0.27)、miR-155水平(2.32±0.31)、Th17细胞(3.85±0.52)%及Th17/Treg比值(1.84±0.17)均高于非EBV感染组,而Treg细胞(2.94±0.31)%低于非EBV感染组(P<0.05)。ROC结果显示,miR-155、Treg细胞、Th17细胞及.Th17/Treg联合检测预测慢性牙周炎EBV感染的曲线下面积(AUC)最高(P<0.05)。结论miR-155、Th17/Treg表达均与慢性牙周炎患者EBV感染有关,且miR-155、Treg细胞、Th17细胞及Th17/Treg联合检测可有效预测慢性牙周炎EBV感染。OBJECTIVE To investigate the effect of Epstein-Barr virus(EBV)infection on the expression of micror-na-155(miR-155)and T-helper cell type 17/regulatory T cells(TH17/Treg)in peripheral blood of patients with chronic periodontitis.METHODS A total of 66 patients with chronic periodontitis admitted to Deyang Stomatologi-cal Hospital from Jan.2022 to Dec.2023 were enrolled and divided into the EBV-infected group with 21 cases and non-EBV group with 45 cases.The levels of miR-155,Treg cells,TH17 cells were measured by real-time fluores-cence quantitative polymerase chain reaction(RT-FQPCR)and flow cytometry,receiver operating characteristic(ROC)curve was used to analyze the predictive value.RESULTS The percentage of smoking history(57.14%),plaque index(2.58±0.27),miR-155level(2.32±0.31),Th17 cells[(3.85±0.52)%] and Th17/Tregratio(1.84±0.17)in EBV infected group were higher than those in non-EBV group(P<0.05),the percentage of Treg cells[(2.94±0.31)%]showed the opposite trend(P<0.05).The area under the curve(AUC)of the combina-tion of miR-155,Treg,Th17 and Th17/Treg was statistically higher than those of the single indicators(P<0.05).CONCLUSION The expressions of miR-155 and Th17/Treg are related to EBV infection in patients with chronic periodontitis,and their combined detection can effectively predict EBV infection for chronic periodontitis.

关 键 词：EB病毒感染 慢性牙周炎 MIR-155 辅助性T细胞17/调节性T细胞 

分 类 号：R781.4[医药卫生—口腔医学]