马钱苷通过circ_0030018/miR-142-5p对口腔鳞癌细胞SCC15增殖、迁移及凋亡的影响  

The effect of loganin on the proliferation,migration and apoptosis of oral squamous cell carcinoma cell line SCC15 through circ_0030018/miR-142-5p

作　　者：崔悦 蔡爽 刘学伟[1] 罗思岳[1] 穆静文 CUI Yue;CAI Shuang;LIU Xuewei;LUO Siyue;MU Jingwen(Department of Stomatology,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China)

机构地区：[1]北京中医药大学东方医院口腔科,北京100078

出　　处：《中南医学科学杂志》2024年第6期906-910,共5页Medical Science Journal of Central South China

摘　　要：目的探讨马钱苷对人口腔鳞癌细胞SCC15增殖、迁移及凋亡的影响及其可能作用机制。方法不同质量浓度的马钱苷处理SCC15细胞,小干扰RNA的正常对照(si-NC)、circ_0030018小干扰RNA(si-circ_0030018)分别转染至SCC15细胞,将pcDNA-circ_0030018转染至SCC15细胞后用马钱苷处理细胞;CCK-8实验检测细胞存活率;qRT-PCR法检测circ_0030018、miR-142-5p的表达;划痕和流式细胞术实验分别检测细胞迁移能力和凋亡率;circ_0030018与miR-142-5p相互作用经双荧光素酶报告实验证实;Western blotting法检测cleaved-Caspase-3蛋白表达量。结果口腔鳞癌组织中circ_0030018的表达量高于癌旁组织(P<0.05),miR-142-5p的表达量低于癌旁组织(P<0.05);马钱苷可降低circ_0030018的表达量、细胞存活率、集落形成数和迁移距离(P<0.05),而提高miR-142-5p的表达量、细胞凋亡率和cleaved-Caspase-3水平(P<0.05);circ_0030018可靶向调控miR-142-5p;转染si-circ_0030018提高了miR-142-5p表达、细胞凋亡率和cleaved-Caspase-3水平(P<0.05),减少了细胞存活率、集落形成数和迁移距离(P<0.05);转染pcDNA-circ_0030018可降低马钱苷对SCC15细胞增殖、迁移及凋亡的作用。结论马钱苷可通过调节circ_0030018/miR-142-5p表达从而抑制口腔鳞癌细胞增殖、克隆形成、迁移及诱导细胞凋亡。Aim To explore the effect of loganin on the proliferation,migration and apoptosis of human oral squamous cell carcinoma cell line SCC15 and its possible mechanism.Methods Different concentrations of loganin were used to treat SCC15 cells,and siRNA normal control(si-NC)and circ_0030018 siRNA(si-circ_0030018)were use to transfect SCC15 cells.After transfecting pcDNA-circ_0030018 into SCC15 cells,the cells were treated with loganin.CCK-8 experiment was used to detect cell viability.qRT-PCR method was used to detect the expression levels of circ_0030018 and microRNA-142-5p(miR-142-5p).Scratch test and Flow cytometry assay were used to detect cell migration ability and apoptosis rate,respectively.The dual luciferase reporter experiment was used to verify the targeting relationship between circ_0030018 and miR-142-5p.Western blotting method was used to detect the expression of cleaved-Caspase-3 protein.Results The expression of circ_0030018 in oral squamous cell carcinoma tissues was higher than that of adjacent tissues(P<0.05),while the expression of miR-142-5p was lower than that of adjacent tissues(P<0.05).Loganin treatment could reduce circ_0030018 expression,cell survival rate,colony formation number,and the migration distance(P<0.05),while increase miR-142-5p expression,cell apoptosis rate,and cleaved-Caspase-3 level(P<0.05).Furthermore,circ_0030018 could target miR-142-5p.Transfection of si-circ_0030018 increased miR-142-5p expression,cell apoptosis rate,and cleaved-Caspase-3 level(P<0.05),while decreased cell survival rate,colony formation number,and migration distance(P<0.05).Transfection of pcDNA-circ_0030018 could reduce the effect of loganin on the proliferation,migration and apoptosis of SCC15 cells.Conclusion Loganin could inhibit the proliferation,clone formation,migration,and induce apoptosis of oral squamous cell carcinoma cells by regulating the expression of circ_0030018/miR-142-5p.

关 键 词：口腔鳞癌 马钱苷 circ_0030018 miR-142-5p 细胞增殖 迁移 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]