基于高效液相指纹图谱和海参皂苷A1含量测定结合化学模式识别评价刺参不同部位差异性  

Differences in Different Parts of Sea Cucumber Based on HPLC Fingerprinting and Holotoxin A1 Content Determination Combined with Chemical Pattern Recognition

作　　者：张晓桐 张君 王野 吴岩强 窦德强[1] ZHANG Xiaotong;ZHANG Jun;WANG Ye;WU Yanqiang;DOU Deqiang(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Dalian Bangchuidao Seafood Co.,Ltd.,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]大连棒棰岛海产股份有限公司,辽宁大连116600

出　　处：《食品科技》2024年第11期300-305,共6页Food Science and Technology

基　　金：辽宁省“揭榜挂帅”科技计划项目(2023020449-JH1/104)。

摘　　要：目的:建立刺参不同部位——体壁、肠、精、卵的高效液相指纹图谱与海参皂苷(Holotoxin)A1含量测定方法,结合化学模式识别技术评价其差异性。方法:采用Agilent ZORBAX SB-C_(18)色谱柱(4.6 mm×150 mm,5μm),以0.1%磷酸水溶液(A)-乙腈(B)为流动相梯度洗脱,流速1.0 mL/min,柱温30℃。对刺参各部位8批次样品进行指纹图谱分析和Holotoxin A1含量测定。采用SPSS 25.0和SIMCA 14.1进行化学模式识别,对刺参不同部位进行差异性评价。结果:刺参体壁指纹图谱共有峰17个,肠28个,精25个,卵29个,且各部位相似度均大于0.9。对照品指认环(亮-异亮)二肽、苯丙氨酸和Holotoxin A1共3个峰,其中Holotoxin A1含量在体壁中最高,其次是肠,精和卵中较低。化学模式识别分析中,各样品被聚为3类;由正交偏最小二乘判别分析(Orthogonal partial least squares-discriminant analysis,OPLS-DA)图可知,肠与精的化学成分较为相近,并筛选出3个差异成分。结论:该研究建立的刺参不同部位高效液相指纹图谱稳定、易行,同时测定Holotoxin A1含量,结合化学模式识别分析,可有效评价刺参各部位的差异性。Objective:To establish high performance liquid chromatography(HPLC)fingerprints from different parts of Apostichopus japonicus including body wall,intestine,sperm and egg with a method for determination of the content of sea cucumber saponin holotoxin A1,and to evaluate their differences using chemical pattern recognition.Methods:An Agilent ZORBAX SB-C18 column(4.6 mm×150 mm,5μm)was used for the separation,with a gradient elution using 0.1%phosphoric acid aqueous solution(A)and acetonitrile(B)as mobile phases,at a flow rate of 1.0 mL/min and a column temperature of 30℃.Eight batches of samples from each part were analyzed for fingerprinting and holotoxin A1 content.Chemical pattern recognition was performed using SPSS 25.0 and SIMCA 14.1 for differential evaluation of the different parts of Apostichopus japonicus.Results:The fingerprints of Apostichopus japonicus body wall showed 17 peaks,28 peaks in intestine,25 peaks in sperm,and 29 peaks in eggs,with similarity greater than 0.9.The control product showed 3 peaks of cyclic(leuco-isoleucine)dipeptide,phenylalanine,and holotoxin A1,of which the content of holotoxin A1 was found to be the highest in the body wall,followed by intestine,and the lower one in sperm and eggs.In the chemical pattern recognition analysis,the samples were clustered into three categories,from the orthogonal partial least squaresdiscriminant analysis(OPLS-DA)plot,it can be seen that the chemical compositions of intestine and sperm were more similar,and three differential components were screened.Conclusion:The method established stable and easy-to-follow HPLC fingerprints for different parts of Apostichopus japonicus,while simultaneously determining the content of holotoxin A1.Combined with chemical pattern recognition analysis,it can effectively evaluate the differences between the various parts of Apostichopus japonicus.

关 键 词：刺参 海参皂苷 指纹图谱 化学模式识别 

分 类 号：TS207.3[轻工技术与工程—食品科学]