白蜡树FcF6′H1基因的密码子偏好性分析  

Codon Bias Analysis of FcF6′H1 Gene in Fraxinus chinensis

作　　者：孙晓春[1] 黄文静[1] 李会容 SUN Xiaochun;HUANG Wenjing;LI Huirong(Co-construction Collaborative Innovation Center for Chineses Medicine Resources Industrialization by Shaanxi&Education Ministry/State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),Shaanxi University of Chinese Medicine,Xianyang 712083,China;School of Chemistry,Biology and Environment,Yuxi Normal University,Yuxi 653100,China)

机构地区：[1]陕西中医药大学陕西中药资源产业化省部共建协同创新中心/秦药特色资源研究开发国家重点实验室(培育),陕西咸阳712083 [2]玉溪师范学院化学生物与环境学院,云南玉溪653100

出　　处：《山西农业科学》2025年第1期35-47,共13页Journal of Shanxi Agricultural Sciences

基　　金：陕西省教育厅青年创新团队建设科研计划项目(21JP031);陕西高校青年创新团队项目(陕教[2019]90号);陕西中医药大学秦药特色资源研究开发重点实验室开放课题(KF202326);陕西中医药大学校级科研课题(2023GP32);云南省科技厅基础研究专项(202101AU070005)。

摘　　要：利用CodonW、EMBOSS在线程序分析白蜡树FcF6'H1基因的密码子偏好性,对芸香科、豆科、伞形科、木犀科以及模式植物拟南芥、烟草和番茄中F6'H1的基因密码子进行聚类分析、中性绘图、ENC-plot分析和PR2-plot偏倚分析,研究影响白蜡树FcF6'H1基因密码子偏好性形成的因素,通过FcF6'H1与模式生物的密码子使用频率比较获得最佳受体。结果表明,白蜡树FcF6'H1的GC、GC1、GC2、GC3和GC12含量分别为0.4312、0.5125、0.3490、0.4321和0.4308,CAI值为0.216,ENC值为57.84,表明白蜡树FcF6'H1基因的偏好性较弱;中性绘图、ENC-plot和PR2-plot偏倚分析结果表明,碱基突变和自然选择均会影响FcF6'H1密码子的偏好性;CDS进化树和RSCU值聚类分析结果不完全一致,但是均表明白蜡树FcF6'H1与木樨榄XM_023028611.1、XM_023036788.1、XM_023034893.1聚为一类,确定了F6'H1基因的6个最优密码子,分别是CUC、AUC、AAG、GAG、UCG和ACA;密码子使用频率分析表明,模式生物中番茄和烟草适合作为遗传转化受体,大肠杆菌表达系统适合作为FcF6'H1的异源表达载体。文章初步阐明了白蜡树FcF6'H1基因密码子的使用规律。In this study,the codon bias analysis of FcF6'H1 gene in Fraxinus chinensis was analyzed using CodonW software and EMBOSS online program,cluster analysis,neutral plot,ENC-plot and PR2-plot of the codon of FcF6'H1 gene in Rutaceae,Leguminosae,Umbelliferae,Oleaceae,Arabidopsis,tobacco and tomato was conduceted,the factors affecting information of the codon bias of the FcF6'H1 gene in Fraxinus chinensis were studied,the best receptors were obtained by comparing the codon usage frequencies of FcF6'H1 and model organisms.The rsults showed that the content of GC、GC1、GC2、GC3,and GC12 of FcF6'H1 in Fraxinus chinensis was 0.4312,0.5125,0.3490,0.4321,and 0.4308,respectively,CAI was 0.216,and ENC was 57.84,indicating weak codon bias of FcF6'H1 in Fraxinus chinensis.The results of neutral analysis,ENC-plot analysis,and PR2-plot analysis indicated that codon bias of FcF6'H1 was affected by base mutation and natural selection.The results of CDS evolutionary tree and RSCU cluster analysis were not completely consistent,but both indicated that FcF6'H1 in Fraxinus chinensis and XM_023028611.1,XM_023036788.1,and XM_023034893.1 of Olea europaea var.sylvestris were aggregated into one category.Six optimal codons of F6'H1 gene were identified,they were CUC,AUC,AAG,GAG、UCG,and ACA.The codon usage frequence analysis showed that in the model plants,tomato tobacco were suitable for the genetic transformation receptors,and the Escherichia coli expression system was suitable for the heterologous expression vector of FcF6'H1.This study preliminarily clarified the codon usage pattern of FcF6'H1 gene in Fraxinus chinensis.

关 键 词：白蜡树 FcF6'H1 密码子偏好性 ENC-plot PR2-plot 异源表达 

分 类 号：S792.41[农业科学—林木遗传育种]