间型脉孢菌JY-05211固态发酵玉米秸秆条件优化  

Optimization of Solid State Fermentation Conditions for Corn Stalks by Neurospora Intermedia

作　　者：付文娟 汪启源 朱英卓 高仕 赖幼纯 李绪锐 陈韵琪 袁景欣 李红梅 李月婷 王庆 FU Wen-juan;WANG Qi-yuan;ZHU Ying-zhuo;GAO Shi;LAI You-chun;LI Xu-rui;CHEN Yun-qi;YUAN Jing-xin;LI Hong-mei;LI Yue-ting;WANG Qing(Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas,Meizhou 514015,China;Meizhou Biological Feed Engineering Technology Research Center,Meizhou 514015,China;College of Life Sciences,Jiaying University,Meizhou 514015,China;Guangdong Daimi Biotechnology Limited Corporation,Meizhou 514300,China)

机构地区：[1]广东省山区特色农业资源保护与精准利用重点实验室,广东梅州514015 [2]梅州市生物饲料工程技术研究中心,广东梅州514015 [3]嘉应学院生命科学学院,广东梅州514015 [4]广东代米生物科技有限公司,广东梅州514300

出　　处：《嘉应学院学报》2024年第6期30-36,共7页Journal of Jiaying University

基　　金：广东省科技专项基金项目(2023A0304013);嘉应学院2022年大学生创新创业项目(国家级202210582012)。

摘　　要：为探究间型脉孢菌JY-05211对秸秆中粗纤维的降解能力,以玉米秸秆为发酵原料,以JY-05211为发酵菌种进行固体发酵,通过单因素及正交试验对发酵培养基组分和培养条件进行优化.结果表明,玉米秸秆固体发酵最适培养基配方为玉米秸秆98.45%、蛋白陈1.5%、氯化钙0.05%;最适培养条件为:料液比1:2.1、培养温度28℃、发酵时间7d,在此条件下发酵玉米秸秆其粗纤维降解率为36.58%.研究为玉米秸秆等的高值化利用提供了思路和方法.To investigate the degradation ability of crude fiber in stalk by Neurospora intermedia JY-05211,the selection and optimization of medium constitutes as well as culture conditions was carried out with corn stalks as main material and JY-05211 as fermentaiton strain using one-factor-at-a-time method combined with orthogonal experiment.As a result,the optimal levels for corn stalks,peptone,calcium chloride,material liquid ratio,culture temperature and culture time were determined as 98.45%,1.5%,0.05%,1:2.1,28 C and 7 d,respectively.Confimatory experiments performed under this conditions yielded a maximam of crude fiber degradation rate 36.58%.This study provides a thought and method for the high value utilization of corn stalks.

关 键 词：间型脉孢菌 固体发酵 玉米秸秆 正交优化 

分 类 号：S186.3[农业科学—农业基础科学]